Summary
An HPLC system has been developed in which 23 phenylthiocarbamyl (PTC) amino acids are distinctly separated. Applying this system, the preceding steps in HPLC amino acid analysis i.e. gas-phase hydrolysis of the protein/peptide sample and the pre-column derivatization, were evaluated and optimized. PTC amino acids in HPLC buffer solution are stable for at least 16 hours if stored at 5°–8°C. The sensitivity of the method is in the low pmol range.
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Janssen, P.S.L., van Nispen, J.W., Melgers, P.A.T.A. et al. HPLC analysis of phenylthiocarbamyl (PTC) amino acids. I. Evaluation and optimization of the procedure. Chromatographia 22, 345–350 (1986). https://doi.org/10.1007/BF02268788
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DOI: https://doi.org/10.1007/BF02268788