Summary
The stationary zone mass transfer coefficient (C5) in the reduced plate height equation has been determined experimentally for an adsorption and a partition system generated on the same column. Longitudinal diffusion terms (B-terms) were measured on these systems by applying the arrested elution method.
The experimentally determined C5 terms for the LLC system are 4–6 times lower than for the LSC system.
B-terms for the LLC system were found to be dependent on capacity ratios and were substantially larger than the B-term for the LSC system, which turned out to be independent of capacity ratios. The obstruction factor (γip) for the intraparticle pore space was estimated by fitting the experimentally determined effective diffusion coefficients according to a mass transfer equation for packed beds. This equation was obtained by transforming a known expression for heat transfer in packed beds.
Knowledge of γp allows the prediction of the magnitude of the stationary zone mass transfer (C5-term) and the comparison between the experimental and predicted values. The agreement is good in the LSC system. In the LLC system the predicted and experimental C5 values for capacity ratios up to k′=2.00 are well matched, whereas the difference becomes larger from k′=3 on.
This paper explains why the efficiency of straight phase LSC systems is often substantially surpassed by analogous LLC systems.
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Crombeen, J.P., Poppe, H. & Kraak, J.C. Diffusion inside particles in HPLC columns. Chromatographia 22, 319–328 (1986). https://doi.org/10.1007/BF02268783
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DOI: https://doi.org/10.1007/BF02268783