Summary
A simplified gas chromatographic method for the rapid determination of morphinn in rat and human blood is described. After single extraction, morphine was reconstituded in trimethylanilinium hydroxide and introduced directly into a gas chromatograph equipped with a nitrogen specific flame ionization detector. Limits of detection were 0.5μg/ml for either water or blood solutions. Analytical recoveries of morphine, added to either water or blood were 88±6 and 90±3%\((\bar x \pm SD)\), respectively, when compared with morphine dissolved in methanol. The between-run coefficients of variation were 5.0 and 5.4% for water and blood, respectively, over the range of 0.5–32.2μg/ml, whereas the within-run coefficients of variation at 1μg/ml were 8.1 and 7.9%, respectively. The application of the method to an in vivo animal experiment is demonstrated.
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H. A. Lloyd, H. M. Fales, P. F. Highet, W. J. A. Vandenheuvel, W. C. Wildman, J. Am. Chem. Soc.,82, 3791 (1960).
L. Kazyak, E. C. Knoblock, Anal. Chem.,35, 1448 (1963).
E. Brochmann-Hanssen, T. Furuya, J. Pharm. Sci.,53, 1549 (1964).
K. D. Parker, C. R. Fontan, P. L. Kirk, Anal. Chem.,35, 356 (1963).
E. Brochmann-Hanssen, C. R. Fontan, J. Chromatog.,19, 296 (1965).
E. Brochmann-Hanssen, C. R. Fontan, J. Chromatog.20, 394 (1965).
C. McMartin, H. V. Street, J. Chromatog.,22, 274 (1966).
H. V. Street, J. Chromatog.,29, 68 (1967).
M. W. Anders, G. J. Mannering, Anal. Chem.,34, 730 (1962).
S. J. Mule, Anal. Chem.36, 1907 (1964).
E. Brochmann-Hanssen A. B. Svendsen, J. Pharm. Sci.,52, 1134 (1963).
G. E. Martin, J. S. Swinehart, Anal. Chem.,38, 1789 (1966).
G. R. Wilkinson, E. L. Way, Biochem. Pharmacol.,18, 1435 (1969).
N. Ikekawa, K. Takayama, E. Hosoya, T. Oka, Anal. Biochem.,28, 156 (1969).
S. Y. Yeh, J. Pharm. Sci.,62, 1827 (1973).
E. Schmerzler, W. Yu, M. I. Hewitt, I. J. Greenblatt, J. Pharm. Sci.,55, 155 (1966).
J. E. Wallace, J. D. Biggs, K. Blum, Clin. Chim. Acta36, 85 (1972).
D. F. Methis, R. D. Budd, Clin. Toxicol.16, 181 (1980).
B. Dahlstrom, L. Paalzow, J. Pharm. Pharmacol.27, 172 (1975).
J. E. Wallace, H. E. Hamilton, K. Blum, C. Petty, Anal. Chem.,46, 2107 (1974).
W. O. R. Ebbighausen, J. H. Mowat, P. Vestergaard, N. S. Kline, Adv. Biochem. Psychophamacol.,7, 135 (1973).
P. P. Hipp, M. R. Eveland, E. R. Meyer, W. R. Sherman T. J. Cicero, J. Pharmacol. Exp. Therap.,196, 642 (1976).
J. E. Wallace, S. C. Harris, M. W. Peek, Anal. Chem.52, 1328 (1980).
C. Kim, T. Kats, J. Anal. Toxicol.,8, 135 (1984).
R. F. Skinner, E. J. Gallaher, J. B. Knight, E. J. Bonelli, J. Forensic Sci.,17, 189 (1972).
R. Mucha, H. Kalant, M. A. Linseman, Pharmacol. Biochem. Behav.,10, 397 (1979).
G. R. Nakamura, In Toxicological Annual Vol. 3,C. L. Winek andS. P. Shanor, eds, Marcel Dekker, New York, 1979, p. 75.
S. Spector, C. W. Parker, Science,168, 1347 (1970).
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Kim, C. Rapid quantitative analysis of morphine in blood by flash-heater methylation and gas chromatography with a nitrogen-specific detector. Chromatographia 22, 303–306 (1986). https://doi.org/10.1007/BF02268779
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DOI: https://doi.org/10.1007/BF02268779