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Evaluation of various bonded-phase materials for the off-line clean-up procedure of urinary 5-hydroxyindolacetic acid prior to its determination by HPLC

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Summary

Determination of serotonin (5-hydroxytryptamine, 5-HT) and its main metabolite, 5-hydroxyindolacetic acid (5-HIAA), became important in clinical chemistry. Commercially available and newly synthetised bonded-phase materials were evaluated in the off-line clean-up procedure of urine samples prior to the determination of 5-HIAA by high-performance liquid chromatography using electrochemical detection. From the point of reproducibility and recovery the best results were obtained employing our newly synthetised material consisting of silica gel support with wide pore diameter (≥20 nm) and large pore volume (≥2.5 cm3 g−1) with a mean (B.E.T.) surface area of 196m2 g−1. It has been modified by mixed chemically bonded C16−C18 phase. The achieved surface carbon coverage was 14.7% C.

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Author notes

  1. B. Buszewski

    Present address: Department of Chemical Physics, Institute of Chemistry, Maria Curie-Skłodowska University, Pl-20 031, Lublin, Poland

Authors and Affiliations

  1. Faculty of Chemical Technology, Slovak Technical University, 812 37, Bratislava, Czechoslovakia

    B. Buszewski & L. Štibrányi

  2. Medical Bionics Research Institute, 833 08, Bratislava, Czechoslovakia

    K. Šebeková

  3. State Hospital, 812 72, Bratislava, Czechoslovakia

    P. Božek

  4. Research Institute of Drugs, 900 01, Modra, Czechoslovakia

    J. Jendrichovský

  5. Polymer Institute, Centrum of Chemical Research, Slovak Academy of Sciences, 842 36, Bratislava, Czechoslovakia

    I. Novák & D. Berek

Authors
  1. B. Buszewski
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  2. K. Šebeková
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  3. P. Božek
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  4. L. Štibrányi
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  5. J. Jendrichovský
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  6. I. Novák
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  7. D. Berek
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Buszewski, B., Šebeková, K., Božek, P. et al. Evaluation of various bonded-phase materials for the off-line clean-up procedure of urinary 5-hydroxyindolacetic acid prior to its determination by HPLC. Chromatographia 22, 299–302 (1986). https://doi.org/10.1007/BF02268778

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  • DOI: https://doi.org/10.1007/BF02268778

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