Chromatographia

, Volume 22, Issue 7–12, pp 283–286 | Cite as

Reversed phase liquid chromatographic assay of FLA 908, a metabolite of remoxipride, in hydrolysed urine by means of a peak compression effect

  • L. B. Nilsson
  • M. Widman
  • B. Bryske
  • D. Westerlund
Originals

Summary

FLA 908 was identified as a metabolite of remoxipride in human urine after enzymatic hydrolysis. The identity was proven by comparison of its retention time in LC and its UV and mass spectra to authentic FLA 908.

The concentration of FLA 908 in human urine was determined using chromatographic conditions where a peak compression effect was obtained. This effect, giving an extremely narrow peak for FLA 908, made it possible to determine low concentrations of the compound in enzymatically hydrolyzed urine. The limit of quantitation was improved more than by a factor of 5 compared to conventional chromatography and the precision was good with a coefficient of variation of <5%.

Less than 1% (0.44–0.91%) of the administered remoxipride dose was found to be excreted as conjugated FLA 908 while only trace amounts (≤0.01%) of nonconjugated FLA 908 were seen.

Key Words

Column liquid chromatography Ion-pair chromatography Peak compression Remoxipride metabolite Conjugate 

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References

  1. [1]
    S. O. Ögren, H. Hall, C. Köhler, O. Magnusson, L. O. Lindbom, K. Ängeby, L. Florvall Eur. J. Pharmacol.,102, 459 (1984).CrossRefPubMedGoogle Scholar
  2. [2]
    M. Widman, B. Bryske, B. Thermander, to be published.Google Scholar
  3. [3]
    T. de Paulis, Y. Kumar, L. Johansson, S. Rämsby, L. Florvall, H. Hall, K. Ängeby-Möller, S. O. Ögren, J. Med. Chem.,28, 1263 (1985).CrossRefPubMedGoogle Scholar
  4. [4]
    L. Nilsson, D. Westerlund, Anal. Chem.,57, 1835 (1985).CrossRefGoogle Scholar
  5. [5]
    L. Gawell, J. Labelled Compd. Radiopharm.,23, 45 (1985).Google Scholar
  6. [6]
    P. -O. Edlund, D. Westerlund, J. Carlqvist, Wu Bo-Liang, Jin Yuhua, Acta Pharm. Suec.,21, 223 (1984).PubMedGoogle Scholar
  7. [7]
    M. Ervik, K. Kylberg-Hanssen, P. -O. Lagerström, J. Chromatogr.,182, 341 (1980).PubMedGoogle Scholar
  8. [8]
    P. Hartvig, N. -O. Ahnfelt, M. Hammarlund, J. Wessman, J. Chromatogr.,127, 173 (1979).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1986

Authors and Affiliations

  • L. B. Nilsson
    • 1
  • M. Widman
    • 2
  • B. Bryske
    • 2
  • D. Westerlund
    • 3
  1. 1.Department of Bioanalytical Chemistry, Research and Development LaboratoriesAstra Alab ABSödertäljeSweden
  2. 2.Department of Drug Metabolism, Research and Development LaboratoriesAstra Alab ABSödertäljeSweden
  3. 3.Department of Analytical Pharmaceutical ChemistryUppsala University Biomedical CenterUppsalaSweden

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