Abstract
Double staining study of nuclei and cell walls inPoria cocos indicated that the hyphal cells were multinucleate and had no clamp connections. Isozyme analysis of alcohol dehydrogenase (ADH) in 52 natural isolates revealed that there were three types of banding patterns: type I, five bands; type II, one slow band; type III, one fast band. Regenerants expressing type-II or type-III ADH-isozyme pattern were obtained from type-I isolates via protoplast manipulation. When the type-II regenerants were mated with the type-III regenerants, hyphae of type-I phenotype appeared. These data indicated that these type-II and type-III regenerants derived from protoplasts of the type-I isolates were primary hyphae. These primary hyphal cells were also multinucleate. Inter-strain mating ofP. cocos was performed and confirmed by ADH-isozyme analysis. Confronting cultures of a type-III regenerant derived from protoplasts of a type-I isolate and a type-II regenerant derived from a type-II isolate resulted in type-I hyphae.
Similar content being viewed by others
Literature cited
Addleman, K. and Archibald, F. 1993. Kraft pulp bleaching and delignification by dikaryons and monokaryons ofTrametes versicolor. Appl. Environ. Microbiol.59: 266–273.
Fox, H. M., Burden, J., Chang, S. and Peberdy, J. F. 1994. Mating-type incompatibility between commercial strains ofLentinula edodes. Exp. Mycol.18: 95–102.
Fritz, C. W. 1954. Fructification ofPoria cocos (Schw.) Wolf on poplar. Can. J. Bot.32: 545–549.
Hino, I. 1937. Fruit-body of “Bukuryo”. J. Jpn. Bot.13: 672–674. (In Japanese.)
Iwase, K., Matsui, S., Taniguchi, T. and Obayashi, A. 1988. Identification of the cell type of hyphae from cultures of single basidiospores and fruit-body tissues of the mycorrhizal fungusTricholoma robustum by double staining of nuclei and septa. Trans. Mycol. Soc. Japan29: 383–390.
Kawabata, H., Magae, Y., Sasaki, T. and Taniguchi, H. 1992. Mating type analysis of monokaryons regenerated from protoplasts ofFlammulina velutipes. Trans. Mycol. Soc. Japan33: 243–247. (In Japanese.)
Kirby, G. C. and Mulley, A. M. 1982. Inheritance of isozyme mobility differences between two races of the head smut of grasses,Ustilago bullata Berk. Bot. Gaz.143: 243–248.
Kobayasi, Y. and Tubaki, K. 1965. Studies on cultural characters and a sexual reproduction of Heterobasidiomycetes. I. Trans. Mycol. Soc. Japan6: 29–36.
Kropp, B. R. and Fortin, J. A. 1985. Formation and regeneration of protoplasts from the ectomycorrhizal basidiomyceteLaccaria bicolor. Can. J. Bot.64: 1224–1226.
Lindsey, J. P. and Gilbertson, R. L. 1977. Some aspects of bipolar heterothallism and other cultural characters ofPoria carnegiea. Mycologia69: 761–772.
Micales, J. A., Bonde, M. R. and Peterson G. L. 1986. The use of isozyme analysis in fungal taxonomy and genetics. Mycotaxon27: 405–449.
Ohmasa, M. and Furukawa, H. 1986. Analysis of esterase and malate dehydrogenase isozymes ofLentinus edodes by isoelectric focusing for the identification and discrimination of stocks. Trans. Mycol. Soc. Japan27: 79–90.
Raper, C. A. 1985. B-mating-type genes influence survival of nuclei separated from heterokaryons ofSchizophyllum. Exp. Mycol.9: 149–160.
Roux, P. and Labarère, J. 1991. Determination of genes and subunit composition of three isozyme activities inAgaricus bitorquis. Mycol. Res.95: 851–860.
Royse, D. J. and May, B. 1982. Use of isozyme variation to identify genotypic classes ofAgaricus brunnescens. Mycologia74: 93–102.
Soltis, D. E. and Soltis, P. S. 1989. “Isozymes in plant biology,” pp. 5–45. Discorides Press, Portland.
Tabata, M. and Hiraoka, N. 1994. Studies on the formation of fruit body and sclerotium inPoria cocos Wolf native to Japan. Natural Medicines48: 18–27. (In Japanese.)
Tominaga, Y. 1987. Study on culture of tuckahoe. I. Germination of fruit body and formation of sclerotium in log culture and wood chip/sawdust bag culture. Hiroshima Agr. Coll.8: 451–463. (In Japanese.)
Weber, G. F. 1929. The occurrence of tuckahoes andPoria cocos in Florida. Mycologia21: 113–130.
Author information
Authors and Affiliations
About this article
Cite this article
Igari, N., Minoura, S. & Okada, M. Multinucleate nature, and mating by use of isozyme analysis inPoria cocos . Mycoscience 36, 405–411 (1995). https://doi.org/10.1007/BF02268624
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02268624