Isolation and identification of (2S)-2-amino-5-chloro-4-hydroxy-5-hexenoic acid from anAmanita of the sectionRoanokenses (Amanitaceae)


A chlorine-containing non-protein amino acid which was recently discovered from the fruit bodies ofAmanita gymnopus (2S)-2-amino-5-chloro-4-hydroxy-5-hexenoic acid, was isolated and crystallized for the first time from the fruit bodies of an unknown member ofAmanita belonging to the sectionRoanokenses, subsectionSolitariae. The results of elementary analyses, determination of optical rotations,1H- and13C-NMR-spectra, and some chemical reactions supported an earlier proposed structure.

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Literature cited

  1. Bas, C. 1969. Morphology and subdivision ofAmanita and a monograph of its sectionLepidella. Persoonia5: 285–579.

    Google Scholar 

  2. Hatanaka, S. I. 1992. Amino acids from mushrooms. Progress in the Chemistry of Organic Natural Products59: 1–144.

    PubMed  Google Scholar 

  3. Hatanaka, S. I., Furukawa, J., Aoki, T., Akatsuka, H. and Nagasawa, E. 1994. (2S)-2-Amino-5-chloro-4-hydroxy-5-hexenoic acid, a new chloroamino acid, and related compounds fromAmanita gymnopus. Mycoscience35: 391–394.

    Article  Google Scholar 

  4. Singer, R. 1986. “The Agaricales in modern taxonomy, 4th ed.,” Koeltz Sci. Books, Koenigstein. 981p.

    Google Scholar 

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Part 24 in the series “Biochemical studies of nitrogen compounds in fungi.” for Part 23, see Hatanaka, S. I. et al. 1994. this journal35: 391–394.

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Hatanaka, SI., Okada, K. & Nagasawa, E. Isolation and identification of (2S)-2-amino-5-chloro-4-hydroxy-5-hexenoic acid from anAmanita of the sectionRoanokenses (Amanitaceae). Mycoscience 36, 395–397 (1995).

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Key Words

  • (2S)-2-amino-5-chloro-4-hydroxy-5-hexenoic acid
  • chloroamino acid
  • non-protein amino acid
  • Amanita sp