, Volume 36, Issue 2, pp 179–185 | Cite as

Studies on turfgrass snow mold caused byTyphula ishikariensis. II. Microscopical observation of infected bentgrass leaves

  • Ko-Ichi Oshiman
  • Issei Kobayashi
  • Haruhiro Shigemitsu
  • Hitoshi Kunoh
Original Papers


Light and transmission electron microscopy revealed thatTyphula ishikariensis penetrated into bentgrass leaves either through cuticles or stomata either by single hyphae or infection cushions formed on host surfaces. Time course study on infected leaves showed that penetration through stomatal subsidiary cells and their adjacent cells seemed to occur earlier than that through epidermal cells located farther from stomata. More than 30% of epidermal cells were infected by 10 days after inoculation. When hyphae penetrated through an intact cuticle of epidermal cells, they seemed to dissolve host cell walls enzymatically at penetration sites. Physical pressure also seemed to be involved in penetration.

Key Words

Turfgrass snow mold Typhula ishikariensis Ultrastructure 


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Literature cited

  1. Araki, H. 1985. Screening techniques for freezing tolerance and snow mold disease resistance in orchardgrass. Res. Bull. Hokkaido Natl. Agric. Exp. Stn.143: 105–114.Google Scholar
  2. Armentrout, V. N. and Downer, A. J. 1987. Infection cushion development byRhizoctonia solani on cotton. Phytopathology77: 619–623.Google Scholar
  3. Chou, C. K. S. 1978. Penetration of young stems ofPinus radiata byDiplodia pinea. Physiol. Plant Pathol.13: 189–192.Google Scholar
  4. Cooper, R. M. 1983. The mechanisms and significance of enzyme degradation of host cell walls by parasites. In: “Biochemical plant pathology,” (ed. by Callow, J. A.), pp. 101–135. John Wiley, New York.Google Scholar
  5. Hashioka, Y., Ikegami, H. and Murase, T. 1968. Fine structure of the rice blast III. The mode of invasion ofPyricularia oryzae into rice epidermal cells. Bull. Fac. Agr. Gifu Univ.26: 23–30.Google Scholar
  6. Iriki, N and Kuwabara, T. 1993. Half diallel analysis of field resistance of winter wheat toTyphula ishikariensis biotype-A in artificially infested plots. Jpn. J. Breed.43: 495–501.Google Scholar
  7. Ishizaki, H., Fujino, M., Kohno, M. and Kunoh, H. 1979. Effects of validamycin A onPellicularia sasakii (Shirai) S. Ito. Bull. Fac. Agr. Mie Univ.59: 1–32.Google Scholar
  8. Koga, H. and Yoshino, R. 1991a. The ultrastructure of rice panicles infected withPyricularia oryzae Cav. Proc. Assoc. Pl. Prot. Hokuriku39: 17–22.Google Scholar
  9. Koga, H. and Yoshino, R. 1991b. The ultrastructure of rice spikelets infected withPyricularia oryzae Cav. Proc. Assoc. Pl. Prot. Hokuriku39: 23–28.Google Scholar
  10. Kousik, C. S., Snow, J. P. and Berggren, G. T. 1994. Factors affecting infection cushion development byRhizoctonia solani AG-1 IA and IB on soybean leaves. Plant Pathology43: 237–244.Google Scholar
  11. Lumsden, R. D. and Dow, R. L. 1973. Histopathology ofSclerotinia sclerotiorum infection of bean. Phytopathology63: 708–715.Google Scholar
  12. Matsumoto, N. 1989. Autecology of the pathogenic species ofTyphula. Res. Bull. Hokkaido Natl. Agric. Exp. Stn.152: 91–162.Google Scholar
  13. Matsumoto, N. and Sato, T. 1982. Significance of polygalacturonase in snow mold diseases. Ann. Phytopath. Soc. Japan48: 124–125. (Abst. in Japanese.)Google Scholar
  14. Matsumoto, N. and Sato, T. 1983. Factors involved in the resistance of timothy and perennial ryegrass toSclerotia borealis andTyphula isikariensis. Res. Bull. Hokkaido Natl. Agric. Exp. Stn.136: 23–30.Google Scholar
  15. McKeen, W. E. and Rimmer, S. R. 1973. Initial penetration process in powdery mildew infection of susceptible barley leaves. Phytopathology63: 1049–1053.Google Scholar
  16. Oshiman, K., Shigemitsu, H. and Kunoh, H. 1993. Studies on the turfgrass snow mold caused byTyphula ishikariensis. 1. A new in vitro bioassay system for screening antagonistic microorganisms and their isolation. J. Jpn. Soc. Turfgrass Sci.22: 21–28.Google Scholar
  17. Reynolds, E. S. 1963. The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol.17: 208–212.CrossRefPubMedGoogle Scholar
  18. Shigemitsu, H., Kohno, M. and Kunoh, H. 1981. Microscopic observation ofCorticium rolfsii sclerotia infected byAspergillus terreus. Trans. Mycol. Soc. Japan22: 383–391.Google Scholar
  19. Shipton, W. A. and Brown, J. F. 1962. A whole-leaf clearing and staining technique to demonstrate host-pathogen relationships of wheat stem rust. Phytopathology52: 1313.Google Scholar
  20. Smiley, R. W., Dernoeden, P. H. and Clarke, B. B. 1992. “Compendium of turfgrass diseases,” APS Press, St. Paul. 49 p.Google Scholar
  21. Spurr, A. R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res.26: 31–43.CrossRefPubMedGoogle Scholar
  22. Takenaka, S. and Yoshino, R. 1987. Penetration ofTyphula incarnata in wheat plants differing in resistance. Ann. Phytopath. Soc. Japan53: 566–569.Google Scholar
  23. Takenaka, S. and Yoshino, R. 1988. Testing of resistance of wheat cultivars to three snow mold diseases by snow mold chamber methods. Proc. Assoc. Pl. Prot. Hokuriku36: 55–60.Google Scholar
  24. Valsangiacomo, C. and Gessler, C. 1988. Role of cuticular membrane in ontogenic and Vf-resistance of apple leaves againstVenturia inaequalis. Phytopathology78: 1066–1069.Google Scholar

Copyright information

© The Mycological Society of Japan 1995

Authors and Affiliations

  • Ko-Ichi Oshiman
    • 1
  • Issei Kobayashi
    • 2
  • Haruhiro Shigemitsu
    • 1
  • Hitoshi Kunoh
    • 2
  1. 1.Sumitomo Forestry Co., Ltd.Tsukuba-city, IbaragiJapan
  2. 2.Laboratory of Plant Pathology, Faculty of BioresourcesMie UniversityTsu-city, MieJapan

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