Mycoscience

, Volume 36, Issue 2, pp 179–185 | Cite as

Studies on turfgrass snow mold caused byTyphula ishikariensis. II. Microscopical observation of infected bentgrass leaves

  • Ko-Ichi Oshiman
  • Issei Kobayashi
  • Haruhiro Shigemitsu
  • Hitoshi Kunoh
Original Papers

Abstract

Light and transmission electron microscopy revealed thatTyphula ishikariensis penetrated into bentgrass leaves either through cuticles or stomata either by single hyphae or infection cushions formed on host surfaces. Time course study on infected leaves showed that penetration through stomatal subsidiary cells and their adjacent cells seemed to occur earlier than that through epidermal cells located farther from stomata. More than 30% of epidermal cells were infected by 10 days after inoculation. When hyphae penetrated through an intact cuticle of epidermal cells, they seemed to dissolve host cell walls enzymatically at penetration sites. Physical pressure also seemed to be involved in penetration.

Key Words

Turfgrass snow mold Typhula ishikariensis Ultrastructure 

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Copyright information

© The Mycological Society of Japan 1995

Authors and Affiliations

  • Ko-Ichi Oshiman
    • 1
  • Issei Kobayashi
    • 2
  • Haruhiro Shigemitsu
    • 1
  • Hitoshi Kunoh
    • 2
  1. 1.Sumitomo Forestry Co., Ltd.Tsukuba-city, IbaragiJapan
  2. 2.Laboratory of Plant Pathology, Faculty of BioresourcesMie UniversityTsu-city, MieJapan

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