Chromatographia

, Volume 18, Issue 2, pp 87–90 | Cite as

Rapid analysis of opium alkaloids by ion-pair high performance liquid chromatography

  • S. Galewsky
  • C. L. Nessler
Originals

Abstract

A new procedure for the rapid analysis of four major opium alkaloids is described using ion-pair, reversedphase high performance liquid chromatography and recently developed radial compression column technology. The solvents were aqueous 10mM potassium perchlorate with 5.0mM n-butylamine (pH 3.0) and pure acetonitrile. A gradient was run for five minutes from 10% to 70% acetonitrile and held at that level for two minutes. Flow rates were 3 ml/min. Two column packing materials were compared with this solvent system: standard C-18 packing and a fully-end-capped C-18 material. Results obtained with the fully-end-capped C-18 packing and the two solvent ion-pair gradient showed elution times under six minutes, with greater sensitivity (<50 nanograms) than previous systems.

Key Words

Opium alkaloids Reversed-phase Ion-pair HPLC 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1983

Authors and Affiliations

  • S. Galewsky
    • 1
  • C. L. Nessler
    • 1
  1. 1.Department of BiologyTexas A & M UniversityCollege StationUSA

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