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Chromatography of proteins and viruses on macroporous silica modified with carbohydrates

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The adsorption and the chromatography of proteins and viruses on macroporoussilicas have been studied. Modification of the silica surfaces with γ-aminopropyltriethoxysilane and then with carbohydrates has eliminated the irreversible adsorption of these biopolymers. The chromatographic separation of the biopolymers has revealed molecular sieve (size exclusion) effects and reversible adsorption. The influence of pH on the elution of proteins and viruses has been demonstrated.

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References

  1. N. V. Akshinskaya, A. V. Kiselev, Yu. S. Nikitin, Zhurn. Fiz. Khim.38, 488 (1964).

    Google Scholar 

  2. N. K. Bebris, A. V. Kiselev, V. Ya. Mokeev, Yu. S. Nikitin, Ya. I. Yashin, Chromatographia4, 93 (1971).

    Google Scholar 

  3. S. P. Zhdanov, Dokl. Akad. Nauk SSSR82, 281 (1952).

    Google Scholar 

  4. W. Haller, J. Chem. Phys.42, 686 (1965).

    Article  Google Scholar 

  5. W. Haller, Nature206, 693 (1965).

    Google Scholar 

  6. A. V. Kiselev, T. D. Khokhlova, Yu. A. Eltekov, 5th International Symposium on Separation Methods: Column Chromatography, Lausanne 1969 (Supplement, Chimia 1970), Ed.E. Kováts, Swiss Chemists Association, Aarau 1970, p. 124.

    Google Scholar 

  7. S. E. Bresler, D. R. Dobychin, A. S. Popov, A. T. Sukhodolova, Molekulyarnaya Biologiya3, 29 (1969).

    Google Scholar 

  8. G. I. Bespalova, S. E. Bresler, N. V. Katushkina, V. M. Kolikov, Voprosy Virusologii16, 112 (1976).

    Google Scholar 

  9. S. E. Bresler, N. V. Katushkina, V. M. Kolikov, J. L. Potokin, G. N. Vinogradskaya, J. Chromatog.130, 275 (1977).

    Article  Google Scholar 

  10. C. C. Shepard, A. Tiselius, Disc. Faraday Soc.7, 275 (1949).

    Article  Google Scholar 

  11. S. E. Bresler, G. I. Bespalova, V. M. Kolikov, S. P. Zhdanov, E. V. Koromaldy, T. Ya. Luzyanina, L. Ya. Reikh, Acta Virol.19, 190 (1975).

    PubMed  Google Scholar 

  12. F. E. Regnier, R. Noel, J. Chromatog. Sci.14, 316 (1976).

    Google Scholar 

  13. R. A. Messing, J. Am. Chem. Soc.91, 2370 (1969).

    Article  Google Scholar 

  14. R. A. Messing, P. F. Weizs, G. Baum, J. Biomed. Mater. Res.3, 425 (1969).

    Article  PubMed  Google Scholar 

  15. Yu. A. Eltekov, A. V. Kiselev, T. D. Khokhlova, Yu. S. Nikitin, Chromatographia6, 187 (1973).

    Google Scholar 

  16. S. H. Chang, K. M. Gooding, F. E. Regnier, J. Chromatogr.125, 103 (1976).

    Article  PubMed  Google Scholar 

  17. O. I. Voroshilova, A. V. Kiselev, Yu. S. Nikitin, Kolloidn. Zh.42, 223 (1979).

    Google Scholar 

  18. A. V. Kiselev, V. M. Kolikov, B. V. Mchedlishvili, Yu. S. Nikitin, T. D. Khokhlova, Dokl. Akad. Nauk SSSR272, 1158 (1983).

    Google Scholar 

  19. E. S. Chukhrai, O. M. Poltorak, Vestnik Mosk. Univ.6, 271 (1973).

    Google Scholar 

  20. P. Roumeliotis, K. K. Unger, J. Chromatogr.218, 535 (1981).

    Article  Google Scholar 

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Kiselev, A.V., Khokhlova, T.D. & Nikitin, Y.S. Chromatography of proteins and viruses on macroporous silica modified with carbohydrates. Chromatographia 18, 69–72 (1984). https://doi.org/10.1007/BF02268459

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