Skip to main content
SpringerLink
Log in

Chromatographic solutions to pharmaceutical analytical problems

  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

Chromatography plays a major role in pharmaceutical analysis since classic methods such as titrimetry or direct spectroscopic analyses do not provide sufficient selectivity and/or detectability. Of various chromatographic methods, HPLC offers good resolution and great versatility in handling compounds that are thermally unstable, polar, and non-volatile at low temperatures. Discussed in this paper is an example of a pharmaceutical compound viz. baclofen [4-amino-3-(p-chlorophenyl)butyric acid], a compound with low UV absorptivitiy, where HPLC provides the desired selectivity and detectability to allow precise determinations in the presence of pharmaceutical excipients. Discussed here also are investigations on mechanism of separations by ion-exchange and ion-pair reversed-phase chromatography.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. S. Ahuja, Am. Chem. Society Meeting, New York, N.Y. August 1991.

  2. S. Ahuja, 1st International Symposium on Chiral Molecules, Paris, France, May 31–June 2, 1988.

  3. A. Walter, personal communication, January 10, 1975.

  4. H. Piskorik, R. Yang, S. Ahuja, G.R. Padmanabhan, personal communication, December 19, 1974.

  5. P.H. Degan, W. Reiss, J. Chromatogr.,117, 399 (1976).

    Google Scholar 

  6. G. Kochak, F. Honc, J. Chromatogr.,310, 319 (1984).

    Google Scholar 

  7. C.G. Swahn, H. Beving, G. Sedvall, J. Chromatogr.,162, 433 (1979).

    Google Scholar 

  8. V. Das Gupta, J. Liq. Chromatogr.10, 749 (1987).

    Google Scholar 

  9. S. Ahuja, P. Liu, J. Smith, Eighth International Symposium on Column Liquid Chromatography, New York, N.Y. May, 1984.

  10. C. Horvath, W. Melander, I. Molnar, P. Molnar, Anal. Chem.49, 2295 (1977).

    Google Scholar 

  11. B.A. Bidlingmeyer, J. Chromatogr. Sci.18, 52 (1980).

    Google Scholar 

  12. J.H. Knox, R.A. Hartwick, J. Chromatogr.204, 3 (1981).

    Google Scholar 

  13. J.J. Stranahan, S.N. Deming, Anal. Chem.54, 2251 (1982).

    Google Scholar 

  14. S. Ahuja, Retention Mechanism Investigations on Ion-Pair Reversed-Phase Chromatography, American Chemical Society Meeting, Miami, April 28, 1985.

  15. J. Ståhlberg, J. Chromatogr.356, 231 (1986).

    Google Scholar 

  16. J. Ståhlberg, Chromatographia24, 820 (1987).

    Google Scholar 

  17. J. Ståhlberg, A. Bartha, J. Chromatogr.456, 253 (1988).

    Google Scholar 

  18. S. Ahuja, Selectivity and Detectability Optimizations in HPLC, Wiley, New York, 1989.

    Google Scholar 

  19. A. Bartha, G. Vigh, J. Ståhlberg, J. Chromatogr.506, 85 (1990).

    Google Scholar 

  20. S. Ahuja, J. Ashman, personal communication, April 8, 1986.

Download references

Author information

Authors and Affiliations

  1. Development Department, Pharmaceutical Division, CIBA-GEIGY Corp., 10901, Suffern, NY, USA

    S. Ahuja

Authors
  1. S. Ahuja
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Dedicated to Professor Leslie S. Ettre on the occasion of his 70th birthday.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ahuja, S. Chromatographic solutions to pharmaceutical analytical problems. Chromatographia 34, 411–416 (1992). https://doi.org/10.1007/BF02268376

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02268376

Key Words

Search

Navigation