Summary
A system for optimizing gas chromatographic separations by computer control in real time is described. The GC-CPU interface controls the high precision sampling valve, temperature controller, flow controller, programmable gain amplifier, and time base. Algorithms for data acquisition, data reduction, and control are outlined. The criteria for optimization of the experimental conditions are based on peak symmetry, resolution, and relative retention. This approach is compared to other modes of control such as precolumn techniques, serial column switching, recycling columns, and library file searching.
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Literature
“Quantitative Gas Chromatography — Fundamentals to Automation”, J. Gas Chromatog.5, 595–646 (1967).
“Computer Automation of Analytical Gas Chromatography”, J. Chromatog. Sci.7, 709–744 (1969).
“Chromatography and Computers ... An Economical Approach for Every Laboratory” J. Chromatog. Sci.9, 705–741 (1971), and J. Chromatog. Sci.10, 1–30 (1972).
“First International Symposium on Computer Chromatography and Associated Techniques”, Chromatographia5, 61–211 (1972).
Gill, J. M., J. Chromatog. Sci.7, 731 (1969).
Annino, R., andBullock, L. E., Adv. Chromatography 1973,A. Zlatkis, ed., University of Houston, Houston, Texas, 1973, p. 67.
Lorenz, L. J., Culp, R. A., andRogers, L. B., Anal. Chem.42, 979 (1970).
Grushka, E., Myers, M. N., andGiddings, J. C., Anal. Chem.42, 21 (1970).
Anderson, A. H., Gibb, T. C., andLittlewood, A. B., Anal. Chem.42, 434 (1970).
Kirmse, D. W., andWesterberg, A. W., Anal. Chem.43, 1035 (1971).
Glenn, T. H., andCram, S. P., J. Chromatog. Sci.8, 46 (1970).
Oberholtzer, J. E., Anal. Chem.39, 959 (1967).
Bowen, B. E., Cram, S. P., Leitner, J. E., andWade, R. L., Anal. Chem.45, 2185 (1973).
Culp, R. A., Lochmuller, C. H., Moreland, A. K., Swingle, R. S., andRogers, L. B., J. Chromatog. Sci.9, 6 (1971).
Goedert, M., andGuiochon, G., Anal. Chem.45, 1188 (1973).
Oberholtzer, J. E., andRogers, L. B., Anal. Chem.41, 1234 (1969).
Swingle, R. S., andRogers, L. B., Anal. Chem.43, 810 (1971).
Burke, M. F., andThurman, R. G., J. Chromatog. Sci.8, 38 (1970).
Thurman, R. G., Mueller, K. A., andBurke, M. F., J. Chromatog. Sci.9, 77 (1971).
Demming, S. N., andKing, P. G., Research/Development25 (5), 22 (1974).
Lawrence Radiation Laboratory, Analytical Chemistry Section Progress Report,J. E. Harrar, ed., University of California, Livermore, Ca., 1967, p. 4.
Root, J. W., Lee, E. K. C., andRowland, F. W., Science143, 676 (1964).
Guiochon, G., Ecole Polytechnique, Paris, France, personal communication, 1972.
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Cram, S.P., Leitner, J.E. Optimization of chromatographic separations by computer control. Chromatographia 7, 567–581 (1974). https://doi.org/10.1007/BF02268343
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DOI: https://doi.org/10.1007/BF02268343