, Volume 26, Issue 1, pp 372–376 | Cite as

A new procedure for simultaneous calibration of separation and axial dispersion in SEC

  • J. Billiani
  • G. Rois
  • K. Lederer


A new computational procedure for the simultaneous calibration of separation and axial dispersion by using SEC coupled with a LALLS detector is presented. Its performance is tested by SEC/LALLS experiments with commercially available samples of broad and narrow polystyrene standards. The peakbroadening parameters were calculated by a novel method which utilizes the different effects of axial dispersion on polymers with narrow and broad molecular weight distribution. The dispersion function was assumed to be gaussian. The variation of the dispersion parameter with molecular weight is in good agreement with reports in the literature. Experimental chromatograms have been corrected for the axial dispersion effect.

Key Words

Size exclusion chromatography Lightscattering Calibration Axial dispersion Dispersion correction 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • J. Billiani
    • 1
  • G. Rois
    • 1
  • K. Lederer
    • 1
  1. 1.Institut für Chemische und Physikalische Technologie der KunststoffeLeobenAustria

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