Chromatographia

, Volume 26, Issue 1, pp 316–320 | Cite as

Analysis of steroids XXXIX. Estimation of the impurity profile of 5α-androst-2-ene-17-one

  • S. Görög
  • A. Laukó
  • B. Herényi
  • A. Georgakis
  • É. Csizér
  • G. Balogh
  • Gy. Gálik
  • S. Mahó
  • Z. Tuba
Article

Summary

The impurity profile of 5α-androst-2-ene-17-one was investigated by using two different capillary gas chromatographic (GC) and a reverse phase high performance liquid chromatographic systems (HPLC). The impurities were identified by retention matching with authentic standards, diode-array UV spectroscopy, on-line gas chromatography-mass spectrometry (GC-MS), off-line HPLC-MS and NMR spectroscopy. The following impurities were identified and quantitated: ring A saturated, isomeric (1-ene, 3-ene, 4-ene, 5-ene) analogues, 3α and 3β-hydroxy as well as 3β-phenyl derivatives.

Key Words

Capillary gas chromatography High performance liquid chromatography GC-MS Impurity profiles 17-oxo-5α-androstenes 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    S. Görög, B. Herényi, J. Chromatogr.400, 177 (1987).Google Scholar
  2. [2]
    S. Görög, B. Herényi, O. Nyéki, I. Schön, L. Kisfaludy, J. Chromatogr.452, 317 (1988).Google Scholar
  3. [3]
    Z. Tuba, Arzneimittelforschung30, 342 (1980).Google Scholar
  4. [4]
    D. N. Kirk, M. P. Hartshorn “Steroid Reaction Mechanisms”, Elsevier, Amsterdam, 1968, p. 101, 111.Google Scholar
  5. [5]
    J. Fajkos, F. Sorm, Coll. Czech. Chem. Commun.24, 3115 (1959).Google Scholar
  6. [6]
    G. Balogh, A. Csehi, S. Görög, A. Laukó, Z. Tuba, in “Advances in Steroid Analysis '84”, S. Görög, ed., Elsevier, Amsterdam, 1985, p. 301–308.Google Scholar
  7. [7]
    G. Bellucci, F. Macchia, V. Malaguzzi, Tetrahedron Lett. 4973 (1966).Google Scholar
  8. [8]
    R. Grignard, A. Kerboul, Analusis10, 423 (1982).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • S. Görög
    • 1
  • A. Laukó
    • 1
  • B. Herényi
    • 1
  • A. Georgakis
    • 1
  • É. Csizér
    • 1
  • G. Balogh
    • 1
  • Gy. Gálik
    • 1
  • S. Mahó
    • 1
  • Z. Tuba
    • 1
  1. 1.Chemical Works of Gedeon Richter Ltd.BudapestHungary

Personalised recommendations