Summary
The impurity profile of 5α-androst-2-ene-17-one was investigated by using two different capillary gas chromatographic (GC) and a reverse phase high performance liquid chromatographic systems (HPLC). The impurities were identified by retention matching with authentic standards, diode-array UV spectroscopy, on-line gas chromatography-mass spectrometry (GC-MS), off-line HPLC-MS and NMR spectroscopy. The following impurities were identified and quantitated: ring A saturated, isomeric (1-ene, 3-ene, 4-ene, 5-ene) analogues, 3α and 3β-hydroxy as well as 3β-phenyl derivatives.
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For Part XXXVIII, see Reference [1]. This paper is Part III in a series of papers “Estimation of Impurity Profiles of Drugs and Related Materials”. For Part II, see Reference [2].
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Görög, S., Laukó, A., Herényi, B. et al. Analysis of steroids XXXIX. Estimation of the impurity profile of 5α-androst-2-ene-17-one. Chromatographia 26, 316–320 (1988). https://doi.org/10.1007/BF02268173
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DOI: https://doi.org/10.1007/BF02268173