Analysis of steroids XXXIX. Estimation of the impurity profile of 5α-androst-2-ene-17-one
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The impurity profile of 5α-androst-2-ene-17-one was investigated by using two different capillary gas chromatographic (GC) and a reverse phase high performance liquid chromatographic systems (HPLC). The impurities were identified by retention matching with authentic standards, diode-array UV spectroscopy, on-line gas chromatography-mass spectrometry (GC-MS), off-line HPLC-MS and NMR spectroscopy. The following impurities were identified and quantitated: ring A saturated, isomeric (1-ene, 3-ene, 4-ene, 5-ene) analogues, 3α and 3β-hydroxy as well as 3β-phenyl derivatives.
Key WordsCapillary gas chromatography High performance liquid chromatography GC-MS Impurity profiles 17-oxo-5α-androstenes
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