Chromatographia

, Volume 26, Issue 1, pp 215–220 | Cite as

Purification of the hydrophilic antibiotics epidermin, gallidermin and nikkomycin Z by preparative reversed-phase HPLC

  • H. -P. Fiedler
  • T. Hörner
  • H. Decker
Article

Summary

Preparative reversed-phase HPLC techniques for the separation of different hydrophilic fermentation products have been developed to shorten the comples and time-consuming isolation procedure. Separations were performed with low adsorptive reversed-phase packings and, additionally with strong acidic volatile modifiers to prevent adsorption, especially of the basic polypeptide antibotics epidermin and gallidermin on the stationary phases. The complex nature of the crude products required a particle size of 10 micron to separate the substances in a purity between 94% and 100%. The loading was limited to 100mg per injection on a 16 mm i.d. column, which corresponds to 4×10−3 g/g stationary phase.

Key Words

preparative HPLC Hydrophilic antibotics Adsorption effects 

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References

  1. [1]
    J. Rivier, R. McClintock, R. Galyean, H. Anderson, J. Chromatogr.288, 303–328 (1984).Google Scholar
  2. [2]
    R. D. Sitrin, P. A. DePhillips, J. J. Dingerdissen, in:G. Pience (Ed.) “Development in Industrial Microbiology”, Vol. 27, pp. 65–75, Soc. Industr. Microbiol., 1987.Google Scholar
  3. [3]
    J. Köhler, D. B. Chase, R. D. Farlee, A. J. Vega, J. J. Kirkkland, J. Chromatogr.352, 275–305 (1986).Google Scholar
  4. [4]
    J. L. Glajach, J. J. Kirkland, J. Köhler, J. Chromatogr.384, 81–90 (1987).Google Scholar
  5. [5]
    J. Köhler, J. J. Kirkland, J. Chromatogr.385, 125–150 (1987).Google Scholar
  6. [6]
    H.-P. Fiedler, T. Hörner, A. Wörn, Chromatographia24, 433–438 (1987).Google Scholar
  7. [7]
    H. Allgaier, G. Jung, R. G. Werner, U. Schneider, H. Zähner, Angew. Chem. Int. Ed. Engl.24, 1051–1053 (1985).Google Scholar
  8. [8]
    R. Kellner, G. Jung, T. Hörner, H. Zähner, F. Götz, Europ. J. Biochem.,177, 53–59 (1988).Google Scholar
  9. [9]
    U. Hähn, H. Hagenmaier, H. Höhne, W. A. König, W. Wolf, H. Zähner, Arch. Mikrobiol.107, 143–160 (1976).Google Scholar
  10. [10]
    H.-P. Fiedler, in:G. H. Wagman andR. Cooper (eds.), “Natural Products Isolation”, J. Chromatogra. Libr., Vol. 43, Elsevier, Amsterdam, 1988, p. 1153–189.Google Scholar
  11. [11]
    T. Hörner, H. Zähner, R. Kellner, G. Jung, Appl. Microbiol. Biotechnol., in press.Google Scholar
  12. [12]
    H.-P. Fiedler, J. Chromatogr.204, 313–318 (1981).Google Scholar
  13. [13]
    H.-P. Fiedler, J. Chromatogr.316, 487–494 (1984).Google Scholar
  14. [14]
    H.-P. Fiedler, S. Gogologoulos, 2nd Int. Symp. Preparative Up Scale Chromatography, Baden-Baden, 1988.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlalgsgesellschaft mbH 1988

Authors and Affiliations

  • H. -P. Fiedler
    • 1
  • T. Hörner
    • 1
  • H. Decker
    • 1
  1. 1.Universität Tübingen, Mikrobiologie ITübingenFRG

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