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Chromatographia

, Volume 26, Issue 1, pp 133–138 | Cite as

HPLC with electrochemical detection of metal-flavonoid-complexes isolated from food

  • G. Weber
Article

Summary

The electrochemical and chromatographic behaviour of flavonoid standards and of flavonoids extracted from food (green tea, black tea and onions) is investigated with respect to metalbinding properties. It is shown that metals such as iron, copper or aluminium are complexed by flavonoids, preferrably by those having an aromatic o-dihydroxy structure. This is confirmed by cyclic voltammetry on HPLC fractions (stopped flow) and by AAS measurement of metals. As the complexing sites of flavonoids are closely related to electrochemical properties, this is used for an indirect detection of metal species at low oxidation potentials. For iron species in particular a sensitive and selective detection is possible. For copper reductive detection can also be used.

Key Words

Column liquid chromatography Electrochemical detection Flavonoids Metal-complexes 

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References

  1. [1]
    A. D. Morton, J. Food Technol.3, 269 (1968).Google Scholar
  2. [2]
    A. J. Shikhande, F. J. Francis, J. Food. Sci.39, 904 (1974).Google Scholar
  3. [3]
    M. Thompson, C. R. Williams, Anal. Chim. Acta85, 375 (1976).Google Scholar
  4. [4]
    G. Cetta, G. Pallavicini, R. Tenni, C. Bisi, Ital. J. Biochem.26, 317 (1977).Google Scholar
  5. [5]
    M. Thompson, C. R. Williams, G. E. P. Elliot, Anal. Chim. Acta85, 375 (1976).Google Scholar
  6. [6]
    D. J. Daigle, E. J. Conkerton, J. Liquid Chromatogr.6, 105 (1983).Google Scholar
  7. [7]
    F. Dondi, G. Blo, Y. D. Kahie, G. Lodi, C. Pietrogrande, P. Reschiglian, Chromatographia25, 423 (1988).Google Scholar
  8. [8]
    S. M. Lunte, J. Chromatogr.384, 371 (1987).Google Scholar
  9. [9]
    G. Weber, G. Schwedt, Fresenius Z. Anal. Chem.316, 594 (1983).Google Scholar
  10. [10]
    G. Weber, Anal. Chim. Acta200, 79 (1987).Google Scholar
  11. [11]
    H. Hajabri, A. L. Lalvin, J. M. Riviello, G. G. Wallace, Anal. Chem.59, 54 (1987).Google Scholar
  12. [12]
    J. N. Barisci, G. G. Wallace, J. M. Riviello, Chromatographia25, 162 (1988).Google Scholar
  13. [13]
    A. M. Bond, R. W. Knight, J. B. Reust, D. J. Tucker, G. G. Wallace, Anal. Chim. Acta182, 47 (1986).Google Scholar
  14. [14]
    R. Eller, G. Weber, Fresenius, Z. Anal. Chem.328, 492 (1987).Google Scholar
  15. [15]
    K. Dallenbach-Tölke, S. Nyiredy, B. Meier, O. Sticher, Planta Medica (1987), 189.Google Scholar
  16. [16]
    S. M. Lunte, K. D. Blankenship, S. A. Read, Analyst113, 99 (1988).Google Scholar
  17. [17]
    P. Van Loo, A. De Bruyn, M. Verzele, Chromatographia 25, 15 (1988).Google Scholar
  18. [18]
    W. A. Mac Crehan, Anal. Chem.53, 74 (1981).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • G. Weber
    • 1
  1. 1.Institut für Spektrochemie und angewandte SpektroskopieDortmund 1FRG

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