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Promotion of respiration by auxin in the induction of cell division in suspension culture

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Abstract

Auxins (IAA, NAA, p-CPA) caused 20–30% promotion of respiration of auxin-dependent cell cultures ofNicotiana tabacum, Glycine max, Taraxacum mongolica, andAtriplex sp. and had small if any effect on respiration of auxin-independent cell cultures ofRubus sp. andScorzonera hispanica. Antiauxin (p-CPIBA) did not affect the respiration. The auxin effect on the respiration of tobacco cells was revealed 10 min after its addition to the suspension and reached a maximum value in 60 min. This stimulation preceded the induction of cell division by auxin. Mitochondria isolated from auxin-treated tobacco cells had greater oxidative and phosphorylative activity than mitochondria from untreated cells. However, isolated mitochondria did not respond to auxin. The inhibitors of respiration (cyanide, monoiodoacetate, malonate, and 2,4-dinitrophenol) eliminated auxin effect on the respiration and cell division. It is concluded that the promotion of respiration is a common event for the auxin effects both on cell extension and on cell division. This promotion is necessary for the induction of cell division and is exerted via direct activation of mitochondriain situ.

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References

  • Anderson PC, Lovrien RE, Brenner ML (1981) Energetics of the response of maize coleoptile tissue to indoleacetic acid. Planta 151:499–505

    Article  CAS  Google Scholar 

  • Auerswald H, Günter G (1972) Der Einfluss von MCPA auf Atmung und Enzymaktivität vonMatricaria- undChenopodium-Kallus. Wiss A Pädag Hochschule Potsdam 16:71–74

    Google Scholar 

  • Audus LJ (1960) Effect of growth regulating substances on respiration. In: Ruhland W (ed) Encyclopedia of plant physiology XII, pt 2. Springer-Verlag, Berlin, pp 360–387

    Google Scholar 

  • Bonner J, Bandurski RS (1952) Studies on the physiology, pharmacology and biochemistry of auxin. Annu Rev Plant Physiol 3:59–86

    Article  Google Scholar 

  • Gamburg KZ (1982) Regulation of cell division by auxin in isolated cultures. In: Wareing PF (ed) Plant growth substances 1982. Academic Press, New York, pp 59–67

    Google Scholar 

  • Gamburg KZ, Osharova LM (1973) Effect of auxin on the mitotic cycle of tobacco cells grown in suspension culture (in Russian). Tsitologia 15:681–689

    CAS  Google Scholar 

  • Key JL, Hanson JB, Bils RF (1960) Effect of 2,4-dichlorophenoxy-acetic acid application on activity and composition of mitochondria from soybean. Plant Physiol 35:177–183

    Article  PubMed  CAS  Google Scholar 

  • Komamine AY, Shimizu T (1975) Changes in some enzymic activities and respiration in the early stage of callus formation in a carrot-root tissue culture. Physiol Plant 33:47–52

    Article  CAS  Google Scholar 

  • Le Tran Binh, Köhler KH, Wolf B (1977) Atmungsuntersuchungen an Geweben vonZea mays—Keimlingen im Verlauf der Kallusbildung. Biol Zbl 96:321–333

    CAS  Google Scholar 

  • Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–276

    PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Poljakoff-Mayber A (1955) The effect of IAA on the oxidative activity of mung bean mitochondria. J Exp Bot 6:321–326

    Article  CAS  Google Scholar 

  • Romani RJ, Ros TJ, Pech JC (1981) Cycloheximide stimulation of cyanide-resistant respiration in suspension cultures of senescent pear fruit cells. Plant Physiol 68:823–826

    Article  PubMed  CAS  Google Scholar 

  • Shvetsov SG, Gamburg KZ (1981) Uptake of 2,4-dichlorophenoxyacetic acid by suspension-cultured maize cells. Biochem Physiol Pflanzen 176:676–686

    CAS  Google Scholar 

  • Shvetsov SG, Gamburg KZ (1982) IAA uptake by isolated plant cells (in Russian). Fisiol Biochim Kult Rast (Kiev) 14:17–22

    CAS  Google Scholar 

  • Switzer CM (1957) Effects of herbicides and related chemicals on oxidation and phosphorylation by isolated soybean mitochondria. Plant Physiol 32:42–44

    Article  PubMed  CAS  Google Scholar 

  • Van der Plas LHW, Wagner MJ (1980) Changes in alternative oxidase activity and other mitochondrial parameters with callus formation by potato tuber tissue discs. Plant Sci Lett 17:207–213

    Article  Google Scholar 

  • Vojnikov VK (1979) The effects of protein synthesis inhibitors on the activity of mitochondria from winter triticale seedlings after cooling (in Russian). Fisiol Rast 26:408–414

    Google Scholar 

Download references

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the USSR Academy of Sciences, 664033, Irkutsk, USSR

    L. A. Leonova, K. Z. Gamburg, V. K. Vojnikov & N. N. Varakina

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  1. L. A. Leonova
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  2. K. Z. Gamburg
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  3. V. K. Vojnikov
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  4. N. N. Varakina
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Leonova, L.A., Gamburg, K.Z., Vojnikov, V.K. et al. Promotion of respiration by auxin in the induction of cell division in suspension culture. J Plant Growth Regul 4, 169–176 (1985). https://doi.org/10.1007/BF02266954

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  • DOI: https://doi.org/10.1007/BF02266954

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