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The development of monoclonal antibodies against the cytokinin zeatin riboside

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Abstract

Seven monoclonal anti-zeatin riboside antibodies were characterized by radioimmunoassay (RIA) and found to measure femtomole (10−15 M) quantities (∼20 pg) of this cytokinin. The antibodies had different measuring ranges defined by the linear portion of the logit/log plots; slopes and intercepts of the line varied considerably between the antibodies. Competitive binding trials againstcis-zeatin riboside (cZR), dihydrozeatin riboside (diHZR), zeatin (Z), and isopentenyl adenosine (iPA) showed differences among the seven antibodies in their cross-reactivities towards these structurally related cytokinins. It was possible to combine selected antibodies to provide a mixture with a predictable measuring range and cross-reactivity; the ability to prepare a highly specific reagent in this manner with well-defined reactivity was noted and differences between monoclonal antibody and polyclonal antiserum probes for measurement of cytokinins were discussed.

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Authors and Affiliations

  1. USDA, ARS

    E. J. Trione & G. M. Banowetz

  2. Dept. of Botany and Plant Pathology University, 97331, Corvallis, Oregon, USA

    E. J. Trione & G. M. Banowetz

  3. Dept. of Crop Science, Oregon State University, 97331, Corvallis, Oregon, USA

    B. B. Krygier & J. M. Kathrein

Authors
  1. E. J. Trione
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  2. B. B. Krygier
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  3. G. M. Banowetz
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  4. J. M. Kathrein
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Additional information

The research was supported by the Agricultural Research Service, U.S. Department of Agriculture, Technical Paper No. 7373 of the Oregon Agricultural Experiment Station. Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the U.S. Department of Agriculture and does not imply approval to the exclusion of other products that may also be available.

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Trione, E.J., Krygier, B.B., Banowetz, G.M. et al. The development of monoclonal antibodies against the cytokinin zeatin riboside. J Plant Growth Regul 4, 101–109 (1985). https://doi.org/10.1007/BF02266948

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  • DOI: https://doi.org/10.1007/BF02266948

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