Advertisement

Effect of glyphosate on indole-3-acetic acid metabolism in tolerant and susceptible plants

  • T. T. Lee
  • T. Dumas
Article

Abstract

A comparison study was conducted on the effect of glyphosate (N-[phosphonomethyl]glycine) on indole-3-[2-14C]acetic acid (IAA) metabolism, ethylene production, and growth of 7-day-old seedlings of different plants. The plants tested were American germander (Teucrium canadense L.), soybean (Glycine max L. Merr.), pea (Pisum sativum L. cv. Alaska and Little marvel), mungbean (Vigna radiata L.), and buckwheat (Fagopyrum esculentum Moench). A spray with 2 mM glyphosate affected IAA metabolism to a varied degree. The induced increase of IAA metabolism was greater in buckwheat, Alaska pea, and mungbean than soybean, Little marvel pea, and American germander. The increased IAA metabolism was correlated with the inhibition of growth and with the decrease of ethylene production.

The natural rate of IAA metabolism was markedly different among the plant species and cultivars tested and appeared to be related to the sensitivity of the plants to glyphosate. American germander and Little marvel pea with high rates of IAA metabolism were more tolerant to glyphosate than buckwheat and Alaska pea, which had low rates of IAA metabolism. Plants with a high natural rate of IAA metabolism were probably less dependent on IAA and thus less susceptible to glyphosate.

Keywords

Glyphosate Ethylene Production Shikimate Pathway Tobacco Callus Butanol Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Abu-Irmaileh BE, Jordan, LS (1978) Some aspects of glyphosate action in purple, nutsedge (Cyperus rotundus). Weed Sci 26:700–703Google Scholar
  2. Amrhein N, Schab J, Steinrücken HC (1980) The mode of action of the herbicide glyphosate. Naturwissenschafter 67:356–357CrossRefGoogle Scholar
  3. Amrhein N, Johänning D, Schab J, Schulz A (1983) Biochemical basis for glyphosate-tolerance in a bacterium and a plant tissue culture. FEBS Lett 157:191–196CrossRefGoogle Scholar
  4. Bassi PK, Spencer MS (1982) Effect of carbon dioxide and light on ethylene production in sunflower plants. Plant Physiol 69:1222–1225PubMedCrossRefGoogle Scholar
  5. Baur, JR, Bovey RW, Veech JA (1977) Growth responses in sorghum and wheat induced by glyphosate, Weed Sci 25:238–240Google Scholar
  6. Berlin J, Witte L (1981) Effects of glyphosate on shikimic acid accumulation in tobacco cell cultures with low and high yields of cinnamoyl putrescines. Z Naturforsch 36c:210–214Google Scholar
  7. Brecke BJ, Duke WB (1980) Effect of glyphosate on intact bean plants (Phaseolus vulgaris L.) and isolated cells. Plant Physiol 66:656–659PubMedCrossRefGoogle Scholar
  8. Caseley JC (1972) The effect of environmental factors on the performance of glyphosate againstAgropyron repens. Proc Br Weed Control Conf 11:641–647Google Scholar
  9. Cole DJ, Dodge AD, Caseley JC (1979) Effects of glyphosate on protein synthesis and phenylalanine metabolism in rhizome buds ofAgropyron repens. Plant Physiol 63(Suppl):96Google Scholar
  10. Comai L, Sen LC, Stalker DM (1983) An altered aroA gene-product confers resistance to the herbicide glyphosate. Science 221:370–371PubMedCrossRefGoogle Scholar
  11. Duke SO, Hoagland RE (1978) Effects of glyphosate on metabolism of phenolic compounds. I. Induction of phenylalanine ammonialyase activity in dark-grown maize roots. Plant Sci Lett 11:85–190Google Scholar
  12. Duke SO, Wauchope RD, Hoagland RE, Wills GD (1983) Influence of glyphosate on uptake and translocation of calcium in soybean seedlings. Weed Res 23:133–139CrossRefGoogle Scholar
  13. Hoagland RE, Duke SO, Elmore D (1978) Effects of glyphosate on metabolism of phenolic compounds. 2. Influence on soluble hydroxyphenolic compound, free amino acid and soluble protein levels in dark-grown maize roots. Plant Sci Lett 13:291–299CrossRefGoogle Scholar
  14. Holländer H, Amrhein N (1980) The site of the inhibition of the shikimate pathway by glyphosate. I. Inhibition by glyphosate of phenylpropanoid systhesis in buckwheat (Fagopyrum esculentum Moench). Plant Physiol 66:823–829PubMedCrossRefGoogle Scholar
  15. Jaworski EG (1972) Mode of action of N-phosphonomethylglycine: Inhibition of aromatic amino acid biosynthesis. J Agric Food Chem 20:1195–1198CrossRefGoogle Scholar
  16. Kitchen LM, Witt WW (1981) Inhibition of chlorophyll accumulation by glyphosate. Weed Sci 29:513–516Google Scholar
  17. Klosterboer AD (1974) Phytotoxicity of glyphosate, MSMA, and paraquat to bearing citrus. Proc South Weed Sci 27:166–169Google Scholar
  18. Lee TT (1980a) Effects of phenolic substances on metabolism of exogenous indole-3-acetic acid in maize stems. Physiol Plant 50:107–112CrossRefGoogle Scholar
  19. Lee TT (1980b) Characteristics of glyphosate inhibition of growth in soybean and tobacco callus cultures. Weed Res 20:365–369CrossRefGoogle Scholar
  20. Lee TT (1981) Effects of glyphosate on synthesis and degradation of chlorophyll in soybean and tobacco cells. Weed Res 21:161–164CrossRefGoogle Scholar
  21. Lee TT (1982a) Mode of action of glyphosate in relation to metabolism of indole-3-acetic acid. Physiol Plant 54:289–294CrossRefGoogle Scholar
  22. Lee TT (1982b) Promotion of indole-3-acetic acid oxidation by glyphosate in tobacco callus tissue. J Plant Growth Regul 1:37–48CrossRefGoogle Scholar
  23. Lee TT (1984) Release of lateral buds from apical dominance by glyphosate in soybean and pea seedlings. J Plant Growth Regul 3:227–235CrossRefGoogle Scholar
  24. Lee TT, Dumas T (1983) Effect of glyphosate on ethylene production in tobacco callus. Plant Physiol 72:855–857PubMedCrossRefGoogle Scholar
  25. Lee TT, Dumas T, Jevnikar JJ (1983) Comparison of the effects of glyphosate and related compounds on indole-3-acetic acid metabolism and ethylene production in tobacco callus. Pest Biochem Physiol 20:354–359CrossRefGoogle Scholar
  26. Nickell LG (1982) Plant growth regulators. Agricultural uses. Springer-Verlag, New York, pp 68–69Google Scholar
  27. Rogers SG, Brand LA, Holder SB, Sharps ES, Brackin MJ (1983) Amplification of the aroA gene fromEscherichia coli results in tolerance to the herbicide glyphosate. Appl Environ Microbiol 46:37–43PubMedGoogle Scholar
  28. Roisch U, Lingens F (1980) The mechanism of action of the herbicideN-(phosphonomethyl)glycine: Its effects on the growth and enzymes of aromatic amino acid biosynthesis inEscherichia coli. Hoppe-Seyler's Z Physiol Chem 361:1049–1058PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • T. T. Lee
    • 1
  • T. Dumas
    • 1
  1. 1.London Research Centre, Agriculture CanadaUniversity Sub Post OfficeLondonCanada

Personalised recommendations