Summary
Six stocks ofTheileria annulata isolated from the Sudan and nine stocks ofT. parva, isolated in Kenya and Malawi were grown in bovine lymphoblastoid cell lines. Lysates prepared from the infected cultures were examined electrophoretically on thin layer starch gels for evidence of glucose phosphate isomerase polymorphism. The six stocks ofT. annulata showed major variations in their parasite enzyme patterns but no variation was detected in nine stocks ofT. parva.
Résumé
Six lots deTheileria annulata isolées au Sondan et neuf lots deTh. parva isolées au Kénya et au Malawi ont été cultivés sur lignées de cellules lymphoblastoîdes. Les lysats préparés à partir des cultures infectées ont été examinés par électrophorèse en couche mince sur gélose pour mettre en évidence le polymorphisme du glucose phosphate isomerase. Des variations importantes dans les types d'enzyme parasitaire ont été décelées dans les six lots deTh. annulata mais aucune dans les neuf lots deTh. parva.
Resumen
Seis cepas deTheileria annulata aisladas en Sudan y nueve cepas deT. parva aisladas en Kenia y Malawi se cultivaron en lineas de células linfoblastoides. Se examinaron electroforéticamente en capas delgadas de gelatina almidón, lisados preparados de cultivos infectados, para determinar el poliformismo de la glucosa fosfato isomerasa. Las seis cepas deT. annulata mostraron variaciones significativas en sus patrones enzimàticos; no se encontraron variaciones en las nueve cepas deT. parva.
This is a preview of subscription content, access via your institution.
References
Allsopp, B. A. & Gibson, W. C. (1983).Annals of Tropical Medicine and Hygiene,77, 168–173.
Brown, C. G. D. (1983). Theileria,In vitro cultivation of Protozoan Parasites. (Ed. J. B. Jensen), CRC Press Inc, Boca Raton, Florida, 312 pp.
Gill, B. S., Bansal, G. C., Bhattacharyulu, Y., Kaur, D. & Singh, A. (1980).Research in Veterinary Science.29, 93–97.
Hoogstraal, H. (1956).African Ixodoidea. Vol. 1. Ticks of the Sudan, Research Report NM 005 050.29.07, US Naval Medical Research Unit No. 3, Cairo, Egypt.
Hopkinson, D. A. (1982). Principles and practical approaches to biochemical taxonomy. Biochemical Characterisation ofLeishmania. Proceedings of a Workshop held at the Pan American Health Organisation, Washington DC, 9–11 December 1980.
Irvin, A. D., Dobbelaere, D. A. E., Mwamachi, D. M., Minami, T., Spooner, P. R. & Ocama, J. G. R. (1983).Research in Veterinary Science,35, 341–346.
Melrose, T. R., Brown, C. G. D. & Sharma, R. D. (1980).Research in Veterinary Science,29, 298–304.
Minami, T., Spooner, P. R., Irvin, A. D., Ocama, J. G. R., Dobbelaere, D. A. E. & Fujinaga, T. (1983).Research in Veterinary Science,35, 334–340.
Musisi, F. L. (1978).Transactions of the Royal Society of Tropical Medicine and Hygiene,72, 146.
Musisi, F. L., Kilgour, V., Brown, C. G. D. & Morzaria, S. P. (1981).Research in Veterinary Science,30, 38–43.
Nelson, R. T. & Hirumi, H. (1981).In vitro cloning ofTheileria-infected bovine lymphoblastoid cells: standardisation and characterisation.Advances in the Control of Theileriosis (Eds. A. D. Irvin, M. P. Cunningham and A. S. Young), Martinus Nijhoff Publishers, The Hague, pp. 120–121.
Nyormoi, O., Bwayo, J. J. & Hirumi, H. (1981).Experimental Parasitology,52, 303–311.
Pinder, M., Kurtti, T. J. & Hirumi, H. (1978). Isolation of macroschizonts and piroplasms ofTheileria parva. Fourth International Congress of Parasitology, Section C, Warsaw, Poland.
Pipano, E. (1977). Basic principles ofT. annulata control in theileriosis: Report of a workshop held in Nairobi, Kenya, 1976. (Eds. J. B. Henson and M. Cunningham), IDRC, Ottawa, Canada.
Radley, D. E., Young, A. S., Brown, C. G. D., Burridge, M. J., Cunningham, M. P., Musisi, F. L. & Purnell, R. E. (1975).Veterinary Parasitology,1, 43–50.
Sergent, E., Donatien, A., Parrot, L., Lestoquard, F. & Delpy, L. (1939).Archives de l'Institut Pasteur d'Algerie,17, 301–309.
Shad-Del, F. (1977). Studies on suspension cultures of lymphoid cells infected withTheileria annulata. PhD Thesis. University of Edinburgh.
Shomein, A. M. (1977). Theileriosis in the Sudan. Theileriosis. (Eds. J. B. Henson and M. Campbell), IDRC, Ottawa, Canada, pp. 46–48.
Van der Meer, P., Uilenberg, G., Van der Bergh, S. G., Spanjer, A. A. M. & Perié, N. M. (1981).The Veterinary Quarterly,3, 61–65.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Melrose, T.R., Brown, C.G.D., Morzaria, S.P. et al. Glucose phosphate isomerase polymorphism intheileria annulata andT. parva . Trop Anim Health Prod 16, 239–245 (1984). https://doi.org/10.1007/BF02265331
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02265331
Keywords
- Public Health
- Glucose
- Phosphate
- Starch
- Thin Layer