Human Genetics

, Volume 88, Issue 6, pp 705–706 | Cite as

Two intragenic polymorphisms of the APC-gene detected by PCR and enzymatic digestion

  • Cornelia Kraus
  • Wolfgang G. Ballhausen
DNA Variants

Summary

The gene causing adenomatous polyposis coli (APC) has recently been cloned. Three intragenic polymorphisms were reported to be detectable by single-strand conformation polymorphism analysis. Here, we describe an assay using polymerase-chain-reaction-based amplification and subsequent enzymatic digestion of genomic sequences to identify polymorphic sites at nucleotide positions 1458 and 5037 of the APC gene.

Keywords

Nucleotide Internal Medicine Genomic Sequence Metabolic Disease Enzymatic Digestion 

References

  1. Groden J, Thliveris A, Samowitz W, Carlson M, Gelbert L, Albertsen H, Joslyn G, Stevens J, Spirio L, Robertson M, Sargeant L, Krapcho K, Wolff E, Burt R, Hughes JP, Warrington J, McPherson J, Wasmuth J, LePaslier D, Abderrahim H, Cohen D, Leppert M, White R (1991) Identification and charactrerization of the familial adenomatous polyposis coli gene. Cell 66:589–600Google Scholar
  2. Joslyn G, Carlson M, Thliveris A, Albertsen H, Gelbert L, Samowitz W, Groden J, Stevens J, Spirio L, Robertson M, Sargeant L, Krapcho K, Wolff E, Burt R, Hughes JP, Warrington J, McPherson J, Wasmuth J, LePaslier D, Abderrahim H, Cohen D, Leppert M, White R (1991) Identification and deletion mutations and three new genes at the familial polyposis locus. Cell 66:601–613Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Cornelia Kraus
    • 1
  • Wolfgang G. Ballhausen
    • 1
  1. 1.Institut für Humangenetik der UniversitätErlangenGermany

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