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Changes in plasma glucagon and insulin associated with fasting in sea bass (Dicentrarchus labrax)

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Abstract

Juvenile sea bass (Dicentrarchus labrax) were fasted for 22 days and changes in plasma insulin, glucagon and glucose levels, as well as glycogen and protein content in liver and muscle were analyzed. Glucagon increased initially on the 4th day of fasting. The glucagon/insulin ratio (G/I) increased from a value of 0.11±0.02 (24h of fasting) to 0.21±0.05 (4th day of fasting). Thereafter, both glucagon and insulin levels decreased and remained at low concentrations until the 22nd day of fasting. Plasma glucose levels fell at the beginning of fasting, stabilized between the 4th and 8th day, and gradually declined during the rest of the experiment. There was a body weight loss of 15% and a significant decrease in both the hepatosomatic index and mesenteric fat. The decrease in the percentage of muscle proteins was not significant, while liver glycogen content showed a sharp decline.

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References cited

  • Buckley, L.J. and Bullow, F.J. 1987. Techniques for the estimation of RNA, DNA and protein in fish.In The Age and Growth of Fish. pp. 245–355. Edited by R.C. Summerfelt and G.E. Hall. The Iowa State University Press, Ames.

    Google Scholar 

  • Cahill, G.F. Jr., Herrera, M.G., Morgan, A.P., Soeldner, J.S., Steinke, J., Levy, P.L., Reich, G.A. Jr. and Kipnis, D.M. 1966. Hormone-fuel interrelationships during fasting. J. Clin. Invest. 45: 1751–1769.

    Google Scholar 

  • Fisher, M., Sherwin, R.S., Hendler, R. and Felig, P. 1976. Kinetics of glucagon in man: effects of starvation. Proc. Nat. Acad.Sci. U.S.A. 73: 1734–1739.

    Google Scholar 

  • Fraga, F. 1956. Determinación de glucógeno en moluscos con el reactivo de antrona. Inv. Pesq. 3: 69–74.

    Google Scholar 

  • Gelfand, R.A. and Sherwin, R.S. 1983. Glucagon and starvation.In Handbook of Experimental Pharmacology. Vol. 66, pp. 223–237. Edited by P.J. Lefebvre. Springer-Verlag, Berlin.

    Google Scholar 

  • Gutiérrez, J., Carrillo, M., Zanuy, S. and Planas, J. 1984. Daily rhythms of insulin and glucose levels in the plasma of sea bassDicentrarchus labrax after experimental feeding. Gen. Comp. Endocrinol. 55: 393–397.

    Google Scholar 

  • Gutiérrez, J., Fernández, J., Blasco, J., Gessé, J.M. and Planas, J. 1986. Plasma glucagon levels in different species of fish. Gen. Comp. Endocrinol. 63: 328–333.

    Google Scholar 

  • Gutiérrez, J., Fernández, J., Carrillo, M., Zanuy, S. and Planas, J. 1987. Annual cycle of plasma insulin and glucose of sea bass.Dicentrarchus labrax. J. Fish Physiol. Biochem. 4: 137–141.

    Google Scholar 

  • Gutiérrez, J., Blasco, J., Navarro, I. and Fernández, J. 1989. Starvation effects on immunoreactive glucagon in carp. Gen. Comp. Endocrinol. 72: 292.

    Google Scholar 

  • Hugget, A.S.G. and Nixon, D.A. 1957. Use of glucose peroxidase and o-dianisidine in determination of blood and urinary glucose. Lancet 2: 368–379.

    Google Scholar 

  • Ince, B.V. and Thorpe, A. 1974. Effects of insulin and of metabolite loading on blood metabolites in the European silver eel. Gen Comp. Endocrinol. 23: 460–471.

    Google Scholar 

  • Love, R.M. 1980. The Chemical Biology of Fishes. Vol. 2. Academic Press, London.

    Google Scholar 

  • Lowry, O.H., Rosenburg, N.J., Farr, A.L. and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275.

    Google Scholar 

  • Marliss, E.B., Aoki, T.T., Unger, R.H., Soldner, S. and Cahill, G.F. Jr. 1970. Glucagon levels and metabolic effects in fasting man. J. Clin. Invest. 49: 2256–2271.

    Google Scholar 

  • Minick, M.C. and Chavin, W. 1972. Effects of vertebrate insulins upon serum FFA and phospholipids levels in the goldfishCarassius auratus L. Comp. Biochem. Physiol. 41A: 791–804.

    Google Scholar 

  • Moon, T.V. and Johnston, I.A. 1979. Starvation and enzyme activities in the skeletal muscles and liver of a teleost fish (Pleuronectes platessa). Biochem. Soc. Trans. 7: 1045–1046.

    Google Scholar 

  • Moon, T.V. 1988. Adaptation, constraint and the function of the gluconeogenic pathway. Can. J. Zool. 66: 1059–1068.

    Google Scholar 

  • Moon, T.W., Foster, G.D. and Plisestkaya, E. 1989. Changes in peptide hormones and liver enzymes in the rainbow trout deprived of food for six weeks. Can. J. Zool. 67: 2189–2193.

    Google Scholar 

  • Ottolenghi, C., Puviani, A.C., Barufaldi, A., Gavioli, M.E. and Brighenti, L. 1988. Glucagon control of glycogenolysis on catfish tissues. Comp. Biochem. Physiol. 90B: 285–290.

    Google Scholar 

  • Patent, G.J. and Foà, P.P. 1971. Radioimmunoassay of insulin in fishes, experimentsin vivo andin vitro. Gen. Comp. Endocrinol. 16: 41–46.

    Google Scholar 

  • Pérez, J., Gutiérrez, J., Carrillo, M. and Zanuy, S. 1988a. Postprandial levels of plasma glucose, insulin and immunoreactive glucagon in sea bass (Dicentrarchus labrax). Inv. Pesq. 52: 585–595.

    Google Scholar 

  • Pérez, J., Zanuy, S. and Carrillo, M. 1988b. Effect of diet and feeding time on daily variations in plasma insulin, hepatic c-AMP and other metabolites in a teleost fish.Dicentrarchus labrax L. Fish Physiol. Biochem. 5: 191–197.

    Google Scholar 

  • Pérez, J., Gutiérrez, J., Carrillo, M., Zanuy, S. and Fernández, J. 1989. Effect of bonito insulin injection on plasma immunoreactive glucagon levels and carbohydrate and lipid metabolism of sea bass (Dicentrarchus labrax). Comp. Biochem. Physiol. 94A: 33–36.

    Google Scholar 

  • Plisestkaya, E.M., Dickhoff, W.W., Paquette, T.L. and Gorbman, A. 1986. The assay of salmon insulin by homologous radioimmunoassay. Fish Physiol. Biochem. 1: 37–43.

    Google Scholar 

  • Plisestkaya, E.M., Sundby, A. and Gorbman, A. 1987. Unorthodox interrelations among pancreatic islet hormones in fish. Am. Zool. 27: 97A.

  • Plisestkaya, E.M., Ottolenghi, C., Sheridan, M.A., Mommsen, T.P. and Gorbman, A. 1989. Metabolic effects of salmon glucagon and glucagon-like peptide in coho and chinook salmon. Gen. Comp. Endocrinol. 73: 205–216.

    Google Scholar 

  • Plisestkaya, E.M. 1990. Endocrine pancreas of teleost fish: A model for interaction of islet hormones? J. Exp. Zool. Suppl. 4: 53–57.

    Google Scholar 

  • Ruderman, N.B. 1975. Muscle amino acid metabolism and gluconeogenesis. Ann. Rev. Med. 26: 245–258.

    Google Scholar 

  • Seitz, H.J., Muller, M.J., Krone, W. and Tarnowsky, W. 1977. Coordinate control of intermediary metabolism in rat liver by the insulin/glucagon ratio during starvation and after glucose refeeding. Regulatory significance of log chain acyl CoA and cyclic AMP. Arch. Biochem. Biophys. 183: 647–663.

    Google Scholar 

  • Sheridan, M.A. and Plisestkaya, E.M. 1988. Effects of nutritional state on lipid and carbohydrate metabolism of coho salmon. Am. Zool. 28: 56A.

  • Stirling, H.P. 1976. Effects of experimental feeding and starvation on the proximate composition of the European bassDicentrarchus labrax. Mar. Biol. 34: 85–91.

    Google Scholar 

  • Thorpe, A. and Ince, B.W. 1976. Plasma insulin levels in teleosts determined by a charcoal-separation radioimmunoassay technique. Gen. Comp. Endocrinol. 30: 332–339.

    Google Scholar 

  • Unger, R.H. and Lefèbre, P.J. 1972. Glucagon physiology.In Glucagon, Molecular Physiology, Clinical and Therapeutic Implications. pp. 213–244. Edited by P.J. Lefèbre and R.H. Unger. Pergamon Press, Oxford.

    Google Scholar 

  • Zammit, V.A. and Newsholme, E.A. 1979. Activities of enzymes of fat and ketone body metabolism and effects of starvation on blood concentrations of glucose and fat fuels in teleost and elasmobranch fish. Biochem. J. 194: 313–322.

    Google Scholar 

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Gutiérrez, J., Pérez, J., Navarro, I. et al. Changes in plasma glucagon and insulin associated with fasting in sea bass (Dicentrarchus labrax). Fish Physiol Biochem 9, 107–112 (1991). https://doi.org/10.1007/BF02265126

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