Summary
An HPLC method has been developed for the determination of SL 85.1016, a new calcium antagonist arylbenzylamide methylthioether derivative. SL 85.1016 and the internal standard, SL 87.0210, are extracted from alkaline human plasma withn-hexane and back extracted into 0.05 M phosphate buffer (pH 2.5; 0.2 ml). Acetonitrile (50 μl) is added to the final aqueous extract in order to prevent absorption of the compounds of interest onto the walls of the glass tube; this solution then is partially processed by HPLC on a C18 column with UV detection (254 nm). The determination limit of the method is 2 ng.ml−1 of SL 85.1016 in human plasma; the response to the drug is linear in the range 2–200 ng.mg−1.
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Flaminio, L., Malavasi, B., Ripamonti, M. et al. Determination of a new calcium antagonist in human plasma by liquid extraction enrichment and HPLC with UV detection. Chromatographia 36, 343–346 (1993). https://doi.org/10.1007/BF02263888
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DOI: https://doi.org/10.1007/BF02263888