Chromatographia

, Volume 36, Issue 1, pp 323–329 | Cite as

Sample stacking effects and large injection volumes in micellar electrokinetic chromatography of ionic compounds: Direct determination of iso-α-acids in beer

  • R. Szücs
  • J. Vindevogel
  • P. Sandra
  • L. C. Verhagen
Article

Summary

The possibility of using large injection volumes in micellar electrokinetic chromatography (MEKC) has been evaluated. With charged compounds effective focusing is observed. For a given liquid sample, the ratio of sample-to-buffer conductivity is decisive in obtaining this effect. The direct analysis of iso-α-acids in beer, thus omitting prior extraction, is demonstrated. Data on quantitative analysis are presented.

Key Words

Micellar electrokinetic chromatography Injection volume On-column sample focusing Iso-α-acid analysis Beer analysis 

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References

  1. [1]
    J. Vindevogel, R. Szücs, P. Sandra, L. C. Verhagen, J. High Res. Chrom.14, 584 (1991).Google Scholar
  2. [2]
    J. Vindevogel, P. Sandra, L. C. Verhagen, J. High Res. Chrom.13, 295 (1990).Google Scholar
  3. [3]
    J. Vindevogel, P. Sandra, J. High Res. Chrom.14, 795 (1991).Google Scholar
  4. [4]
    J. Vindevogel, R. Szücs, P. Sandra, L. C. Verhagen, Proc. 13th Int. Symp. on Capillary Chromatography, Riva del Garda (May, 1991), 1212.Google Scholar
  5. [5]
    I. McMurrough, M. V. Lynch, F. Murray, M. Kearny, F. Nitzsche, J. Am. Soc. Brew. Chem.45, 6 (1987).Google Scholar
  6. [6]
    I. McMurrough, J. Byrne, E. Collins, J. Cooney, P. James, J. Am. Soc. Brew. Chem.46, 51 (1988).Google Scholar
  7. [7]
    D. S. Burgi, R.-L. Chien, Anal. Chem.63, 2042 (1991).Google Scholar
  8. [8]
    R.-L. Chien, Anal. Chem.63, 2866 (1991).Google Scholar
  9. [9]
    D. S. Burgi, R.-L. Chien, J. Microcol. Sep.3, 199 (1991).Google Scholar
  10. [10]
    R.-L. Chien, D. S. Burgi, J. Chromatogr.559, 141 (1991).Google Scholar
  11. [11]
    R.-L. Chien, D. S. Burgi, J. Chromatogr.559, 153 (1991).Google Scholar
  12. [12]
    R.-L. Chien, D. S. Burgi, Anal. Chem.64, 489A (1992).Google Scholar
  13. [13]
    R.-L. Chien, D. S. Burgi, Anal. Biochem.202, 306 (1992).Google Scholar
  14. [14]
    R.-L. Chien, D. S. Burgi, Anal. Chem.64, 1046 (1992).Google Scholar
  15. [15]
    A. Vinther, H. Søeberg, J. Chromatogr.559, 3 (1991).Google Scholar
  16. [16]
    E. Grushka, R. M. McCormick, J. Chromatogr.471, 421 (1989).Google Scholar
  17. [17]
    S. Terabe, K. Otsuka, T. Ando, Anal. Chem.57, 834 (1985).Google Scholar
  18. [18]
    R. Weinberger, E. Sapp, S. Moring, J. Chromatogr.516, 271 (1990).Google Scholar
  19. [19]
    R.-L. Chien, J. C. Helmer, Anal. Chem.63, 1354 (1991).Google Scholar
  20. [20]
    W. Nashabeh, Z. El Rassi, J. High Res. Chrom.15, 289 (1992).Google Scholar
  21. [21]
    S. Terabe, K. Otsuka, T. Ando, Anal. Chem.61, 251 (1989).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1993

Authors and Affiliations

  • R. Szücs
    • 1
  • J. Vindevogel
    • 1
  • P. Sandra
    • 1
  • L. C. Verhagen
    • 2
  1. 1.Laboratory of Organic ChemistryUniversity of GentGentBelgium
  2. 2.Heineken Technical ServicesZoeterwoudeThe Netherlands

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