Summary
Chlortoluron and its metabolites have been determined in cereals by gas chromatography. Chlortoluron is pyrolyzed to the corresponding phenylisocyanate, which is determined by GC with nitrogen-phosphorous detection (NPD) and confirmed by GC with ion trap detection (ITD).N-demethyl metabolites of chlortoluron are determined, after conversion to the perfluoroacyl derivatives, by GC with ion trap detection by single ion monitoring;N-ethyl-p-chloroacetanilide is used as internal standard. The hydroxymethyl metabolite, mainly present as β-glucoside, can be analyzed, after enzymatic hydrolysis with β-glucosidase and conversion to the ethyl derivative, by GC with ion trap detection by SIM;N-ethylchlortoluron is used as internal standard.
The methods proposed are reproducible and sensitive enough for determination of these compounds at residue levels in cereals.
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Pérez, S., Matienzo, M.T. & Tadeo, J.L. GC-ITD analysis of chlortoluron and its metabolites in cereals. Chromatographia 36, 195–200 (1993). https://doi.org/10.1007/BF02263862
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DOI: https://doi.org/10.1007/BF02263862