Skip to main content
Log in

Effect of drying temperature and blanching on the degradation of chlorophyll a and b in mint (Mentha spicata Huds.) and basil (Ocimum basilicum): Analysis by high performance liquid chromatography with photodiode array detection

  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

The effect of drying conditions on the preservation of chlorophyll pigments in mint and basil have been investigated in order to determine the effects of drying temperature and whether or not there was a prior blanching.

Pigments extracted from fresh and dried samples were analyzed by reversed phase high performance liquid chromatography coupled to a photodiode array detector; isocratic separation was performed on a Zorbax ODS C18 column.

The purity of the chromatographic peaks of chlorophylls and breakdown products was investigated. The visible spectra of standard samples of chlorophylls and pheophytins were compared, using least squares normalization with those of peaks from the extracts of fresh and dried mint and basil. The study has shown that chlorophylls were better preserved when drying was preceded by a short blanching; if samples were not blanched before drying, the degradation of chlorophylls a and b was best prevented by drying at low temperatures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. F.J. Francis, in: Sensory properties of Foods, Colour and appearance as dominating sensory properties of foods. Eds. G.G. Birch, J.G. Brennan, K.J. Parker. Applied Science Publishers LTD London (1977)

    Google Scholar 

  2. J.C. Cheftel, H. Cheftel, Introduction à la biochimie et à la technologie des aliments. Technique et Documentation, Société P.I.C. Genève (1976)

    Google Scholar 

  3. T.P. Coultate, Food — The chemistry of its components, The Royal Society of Chemistry, London (1984)

    Google Scholar 

  4. C. Marty-Audouin, A. Lebert, T. Rocha, in: Drying of Solids, Influence of drying on the color of plant products,A. Mujumdar, ed., Oxford & IBH Publishing Co. New Delhi, India (1992)

    Google Scholar 

  5. C. Marty-Audouin, A. Lebert, T. Rocha, Studies on the color of sweet mint, EUROFOOD Chemistry 1991, Hamburg, Germany (1991)

  6. L.P. Vernon, Anal. Chem.,32, 1144 (1960)

    Google Scholar 

  7. S.J. Schwartz, J.H. von Elbe, J. Food Sci.,48, 1303 (1983)

    Google Scholar 

  8. F. Khachik, G. Beecher, N. Whittaker, J. Agric. Food Chem.,34, 603 (1986)

    Google Scholar 

  9. S.J. Schwartz, S.L. Woo, J.H. von Elbe, J. Agric. Food Chem.,29, 533 (1981)

    Google Scholar 

  10. A. Lebert, P. Tharrault, T. Rocha, C. Marty-Audouint, J. Fd. Eng.,17, 15 (1992)

    Google Scholar 

  11. S.J. Schwartz, T.V. Lorenzo, J. Food Sci.,56, 1059 (1991)

    Google Scholar 

  12. C.T. Tan, F.J. Francis, J. Food Sci.,27, 232 (1962)

    Google Scholar 

  13. S.H. Schanderl, C.O. Chichester, G.L. Marsh, J. Org. Chem.,27, 2865 (1962)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rocha, T., Marty-Audouin, C. & Lebert, A. Effect of drying temperature and blanching on the degradation of chlorophyll a and b in mint (Mentha spicata Huds.) and basil (Ocimum basilicum): Analysis by high performance liquid chromatography with photodiode array detection. Chromatographia 36, 152–156 (1993). https://doi.org/10.1007/BF02263853

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02263853

Key Words

Navigation