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Urinary hydroxylysine glycosides: Solid phase extraction and reversed phase chromatographic measurement

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Summary

A method is described for measurement of urinary hydroxylysine glycosides (galactosyl-hydroxylysine, or GHYL, and glucosyl-galactosyl-hydroxylysine, or GGHYL) by reversed phase chromatography after purification of the sample by solid phase extraction. Urinary levels of hydroxylysine glycosides has been correlated with collagen catabolism, and the urinary GGHYL: GHYL ratio is indicative of the metabolism of connective tissue. The GGHYL:GHYL ratio might, therefore, be a promising indicator of bone mass loss. The method described is analytically reliable and enables the measurement of a possible marker for diagnosis and follow-up of osteoporosis.

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Authors and Affiliations

  1. Laboratorio Techniche Separative Cromatografiche, Istituto Scientifico H. S. Raffale-DSNP, Via Prinetti 29, 20127, Milano, Italy

    E. Casari, M. Murone & V. Grazioli

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  1. E. Casari
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  2. M. Murone
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  3. V. Grazioli
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Casari, E., Murone, M. & Grazioli, V. Urinary hydroxylysine glycosides: Solid phase extraction and reversed phase chromatographic measurement. Chromatographia 36, 144–146 (1993). https://doi.org/10.1007/BF02263851

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  • DOI: https://doi.org/10.1007/BF02263851

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