Summary
The separation of two families of plastics additives (phenolic antioxidants and UV absorbers) has been achieved by high performance liquid chromatography with a quaternary gradient. A methodology of separation based on a sequential optimization is described. After a preliminary study of the effects of solvents on retention of compounds, the mobile phase is first chosen for each class of additives (Irganox and Tinuvin), then for the mixture of all the compounds and the separation is finally optimized. The importance of the column phase ratio is also reported. The use of two detectors, UV absorbance and light-scattering, enables all the compounds to be detected. The performance of the detectors has been compared and the effects of the nebulization temperature on the detection of low molecular mass compounds is reported.
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Lesellier, E., Tchapla, A. Sequential optimisation of the separation of a complex mixture of plastics additives by HPLC with a quaternary gradient and a dual detection system. Chromatographia 36, 135–143 (1993). https://doi.org/10.1007/BF02263850
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DOI: https://doi.org/10.1007/BF02263850