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Chromatographic determination of position and configuration isomers of methyl oleate hydroxides from corresponding hydroperoxides

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Summary

Studies of lipid oxidation usually employ such model systems as purified fatty-acid methyl ester. While methyl oleate hydroperoxides (MOPHs) can only be readily separated from the matrix by HPLC, because of their heat-susceptibility and relative instability, these same techniques are unable to separatecis MOHP fromtrans isomers. The present study reports an enhanced, rapid separation method forcis andtrans isomers of methyl oleate hydroxides, as well as HPLC determination of positional isomers per fraction of configuration isomer and isomer identification by gas chromatography-mass spectrometry.

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Authors and Affiliations

  1. Dipartimento di Tecnologia, Chimica e Microbiologia Agroalimentare, Università di Udine, Via Marangoni 97, 33100, Udine, Italy

    R. Bortolomeazzi & L. Pizzale

  2. D.I.S.T.A.M., Università di Firenze, Via Donizetti 6, 50144, Firenze, Italy

    G. Lercker

Authors
  1. R. Bortolomeazzi
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  2. L. Pizzale
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  3. G. Lercker
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Bortolomeazzi, R., Pizzale, L. & Lercker, G. Chromatographic determination of position and configuration isomers of methyl oleate hydroxides from corresponding hydroperoxides. Chromatographia 36, 61–64 (1993). https://doi.org/10.1007/BF02263838

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  • DOI: https://doi.org/10.1007/BF02263838

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