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Chromosome Research

, Volume 4, Issue 4, pp 310–313 | Cite as

Mapping of a new MAP kinase activated protein kinase gene (3PK) to human chromosome band 3p21.2 and ordering of 3PK and two cosmid markers in the 3p22–p21 tumour-suppressor region by two-colour fluorescencein situ hybridization

  • Anna Szeles
  • Svetlana Bajalica-Lagercrantz
  • Annika Lindblom
  • Tamara Lushnikova
  • Vladimir I. Kashuba
  • Stephan Imreh
  • Magnus Nordenskjöld
  • George Klein
  • R. Zabarovsky
Article

Abstract

ANotl-linking clone NL1-210 (D3S1656) that contains the human MAP kinase activated protein kinase (3PK) gene was localized to 3p21.2 on DAPI-banded and propidium iodide (R-bands)-stained chromosomes by fluorescencein situ hybridization (FISH). For more precise localization of 3PK, two cosmid probes were used as a frame. In order to establish this frame, twoNotl-linking clones, NL2-008 (D3S1648) and NL3-003 (D3S3872) were used to screen the cosmid library for locus extension. They mapped to 3p21 and were found to belong to two separate contigs ofNotl-jumping and linking clones. Using FISH on DAPI-banded metaphase chromosomes, we have determined the precise localization of cosNL2-008 and cosNL3-003 to 3p21.2–p21.1 and 3p22–p21.3 respectively. The 3PK gene was localized to the 3p21.2 region within this frame by two-colour FISH. The orientation of the probes are tel-D3S3872-3PK-D3S1648-cen.

Keywords

FISH human chromosome 3 mapping 3PKD351656 

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Copyright information

© Rapid Science Publishers 1996

Authors and Affiliations

  • Anna Szeles
    • 1
  • Svetlana Bajalica-Lagercrantz
    • 2
  • Annika Lindblom
    • 2
  • Tamara Lushnikova
    • 3
  • Vladimir I. Kashuba
    • 1
  • Stephan Imreh
    • 1
  • Magnus Nordenskjöld
    • 2
  • George Klein
    • 1
  • R. Zabarovsky
    • 1
  1. 1.Microbiology and Tumor Biology Center (MTC)Karolinska InstitutetStockholmSweden
  2. 2.Department of Clinical GeneticsKarolinska HospitalStockholmSweden
  3. 3.Laboratory of Molecular GeneticsInstitute of Chemical Physics and BiophysicsTallinnEstonia

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