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A new enzymatic method for pyridoxal-5-phosphate determination

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Journal of Inherited Metabolic Disease

Abstract

An improved method for the determination of pyridoxal-5-phosphate using tyrosine decarboxylase is described.

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References

  • Bhagavan, H. N., Koogler, J. M. and Coursin, D. B. Enzymatic microassay of pyridoxal-5-phosphate usingl-tyrosine decarboxylase andl-[1-14C]tyrosine.Int. J. Vitam. Nutr. Res. 46 (1976) 160–164

    Google Scholar 

  • Chabner, B. and Livingston, D. A simple enzymic assay for pyridoxal phosphate.Anal. Biochem. 34 (1970) 413–423

    Google Scholar 

  • Epps, H. M. R. Properties ofl(-)-tyrosine decarboxylase.Biochem. J. 38 (1944) 245–249

    Google Scholar 

  • Reinken, L. Eine Mikromethode zur Bestimmung von Pyridoxal-5-phosphat im Serum mittels Decarboxylierung vonl-Tyrosine-l-14C.Internat. Z. Vit. Ern. Forschung 42 (1972) 477–481

    Google Scholar 

  • Shin-Buehring, Y., Osang, M., Ziegler, R. and Schaub, J. A method for galactose-l-phosphate uridyltransferase assay and the separation of its isozyme by DEAE-cellulose column chromatography.Clin. Chim. Acta 70 (1976) 371–377

    Google Scholar 

  • Shin-Buehring, Y., Osang, M., Ziegler, R. and Schaub, J. A simple assay for galactokinase using DEAE-cellulose column chromatography.Clin. Chim. Acta 74 (1977) 1–5

    Google Scholar 

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Authors and Affiliations

  1. Children's Hospital, University of Munich, Lindwurmstrasse 4, D-8000, München 2, FRG

    Y. S. Shin-Buehring, R. Rasshofer & W. Endres

Authors
  1. Y. S. Shin-Buehring
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  2. R. Rasshofer
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  3. W. Endres
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Shin-Buehring, Y.S., Rasshofer, R. & Endres, W. A new enzymatic method for pyridoxal-5-phosphate determination. J Inherit Metab Dis 4, 123–124 (1981). https://doi.org/10.1007/BF02263621

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  • DOI: https://doi.org/10.1007/BF02263621

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