Abstract
Isotachophoresis provides an effective method of examining purines and pyrimidines in muscle extracts. Its application to dystrophic muscle of the Duchenne type is illustrated.
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Castro-Gago, M., Jimenez, J. F., Pombo, M., Tojo, Rl., Couselo, J. M. and Pena, J. Allopurinol in Duchenne muscular dystrophy.Lancet 1 (1980) 1358–1359
De Bruyn, C. H. M. M., Kulakowski, S., van Bennekom, C. A., Renoirte, P. and Müller, M. M. Purine metabolism in Duchenne muscular dystrophy.Adv. Exp. Med. Biol. 122A (1980) 177–182
Everaerts, F. M., Beckers, J. L. and Verheggen, P. E. M.Isotachophoresis: Theory, Instrumentation and Applications. Elsevier Scientific Publishing Company, Amsterdam, 1976
Gower, D. C. and Woledge, R. C. The use of isotachophoresis for analysis of muscle extracts.Sci. Tools 24 (1977) 17–21
Kar, N. C. and Pearson, C. M. Muscle adenylic acid deaminase activity.Neurology 23 (1973a) 479–482
Kar, N. C. and Pearson, C. M. 5′-Nucelotidase activity of normal and dystrophic human muscle.Proc. Soc. Exp. Biol. Med. 143 (1973b) 1125–1126
Kulakowski, S., De Bruyn, C. H. M. M. and Renoirte, P. Dynamometric and biochemical observations in Duchenne patients receiving allopurinol.Neuropädiat. (1981) (in press)
Mendell, J. R. and Wiechers, D. O. Lack of benefit of allopurinol in Duchenne dystrophy.Muscle Nerve 2 (1979) 53–56
Neerunjun, J. S., Allsop, J. and Dubowitz, V. Hypoxanthine-guanine phosphoribosyl transferase activity of blood and muscle in Duchenne dystrophy.Muscle Nerve 2 (1979) 19–23
Oerlemans, F., Verheggen, Th., Mikkers, F., Everaerts, F. and De Bruyn, C. Isotachophoretic separation of serum purines and pyrimidines. In Adam, A. and Schots, C. (eds.)Biochemical and Biological Applications of Isotachophoresis. Elsevier Scientific Publishing Company, Amsterdam, 1980, pp. 63–68
Sahota, A., Simmonds, H. A. and Payne, R. H. Separation of urinary purines and pyrimidines by isotachophoresis.J. Pharm. Meth. 2 (1979) 303–312
Solomons, C. C., Ringel, S. P., Nwuke, E. I. and Suga, H. Abnormal adenine metabolism of erythrocytes in Duchenne and myotonic muscular dystrophy.Nature 268 (1977) 55–56
Thomson, W. H. S. and Smith, I. X-linked recessive (Duchenne's) muscular dystrophy and purine metabolism.Lancet 2 (1976) 805–806
Verheggen, Th., Mikkers, F., Everaerts, F., Oerlemans, F. and De Bruyn, C. H. M. M. Determination of uric acid in serum using isotachophoresis.J. Chromatogr. 182 (1980) 317–324
Vignos, P. J. and Warner, J. L. Glycogen, creatine and high energy phosphate in human muscle disease.J. Lab. Clin. Med. 62 (1963) 579–590
Walton, J. N.Disorders of Voluntary Muscle. Churchill Livingstone, Edinburgh and London, 1974
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Oerlemans, F., van Bennekom, C., De Bruyn, C. et al. Metabolite profiling of human muscle extracts by isotachophoresis. J Inherit Metab Dis 4, 109–110 (1981). https://doi.org/10.1007/BF02263615
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DOI: https://doi.org/10.1007/BF02263615