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Metabolite profiling of human muscle extracts by isotachophoresis

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Journal of Inherited Metabolic Disease

Abstract

Isotachophoresis provides an effective method of examining purines and pyrimidines in muscle extracts. Its application to dystrophic muscle of the Duchenne type is illustrated.

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References

  • Castro-Gago, M., Jimenez, J. F., Pombo, M., Tojo, Rl., Couselo, J. M. and Pena, J. Allopurinol in Duchenne muscular dystrophy.Lancet 1 (1980) 1358–1359

    Google Scholar 

  • De Bruyn, C. H. M. M., Kulakowski, S., van Bennekom, C. A., Renoirte, P. and Müller, M. M. Purine metabolism in Duchenne muscular dystrophy.Adv. Exp. Med. Biol. 122A (1980) 177–182

    Google Scholar 

  • Everaerts, F. M., Beckers, J. L. and Verheggen, P. E. M.Isotachophoresis: Theory, Instrumentation and Applications. Elsevier Scientific Publishing Company, Amsterdam, 1976

    Google Scholar 

  • Gower, D. C. and Woledge, R. C. The use of isotachophoresis for analysis of muscle extracts.Sci. Tools 24 (1977) 17–21

    Google Scholar 

  • Kar, N. C. and Pearson, C. M. Muscle adenylic acid deaminase activity.Neurology 23 (1973a) 479–482

    Google Scholar 

  • Kar, N. C. and Pearson, C. M. 5′-Nucelotidase activity of normal and dystrophic human muscle.Proc. Soc. Exp. Biol. Med. 143 (1973b) 1125–1126

    Google Scholar 

  • Kulakowski, S., De Bruyn, C. H. M. M. and Renoirte, P. Dynamometric and biochemical observations in Duchenne patients receiving allopurinol.Neuropädiat. (1981) (in press)

  • Mendell, J. R. and Wiechers, D. O. Lack of benefit of allopurinol in Duchenne dystrophy.Muscle Nerve 2 (1979) 53–56

    Google Scholar 

  • Neerunjun, J. S., Allsop, J. and Dubowitz, V. Hypoxanthine-guanine phosphoribosyl transferase activity of blood and muscle in Duchenne dystrophy.Muscle Nerve 2 (1979) 19–23

    Google Scholar 

  • Oerlemans, F., Verheggen, Th., Mikkers, F., Everaerts, F. and De Bruyn, C. Isotachophoretic separation of serum purines and pyrimidines. In Adam, A. and Schots, C. (eds.)Biochemical and Biological Applications of Isotachophoresis. Elsevier Scientific Publishing Company, Amsterdam, 1980, pp. 63–68

    Google Scholar 

  • Sahota, A., Simmonds, H. A. and Payne, R. H. Separation of urinary purines and pyrimidines by isotachophoresis.J. Pharm. Meth. 2 (1979) 303–312

    Google Scholar 

  • Solomons, C. C., Ringel, S. P., Nwuke, E. I. and Suga, H. Abnormal adenine metabolism of erythrocytes in Duchenne and myotonic muscular dystrophy.Nature 268 (1977) 55–56

    Google Scholar 

  • Thomson, W. H. S. and Smith, I. X-linked recessive (Duchenne's) muscular dystrophy and purine metabolism.Lancet 2 (1976) 805–806

    Google Scholar 

  • Verheggen, Th., Mikkers, F., Everaerts, F., Oerlemans, F. and De Bruyn, C. H. M. M. Determination of uric acid in serum using isotachophoresis.J. Chromatogr. 182 (1980) 317–324

    Google Scholar 

  • Vignos, P. J. and Warner, J. L. Glycogen, creatine and high energy phosphate in human muscle disease.J. Lab. Clin. Med. 62 (1963) 579–590

    Google Scholar 

  • Walton, J. N.Disorders of Voluntary Muscle. Churchill Livingstone, Edinburgh and London, 1974

    Google Scholar 

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Oerlemans, F., van Bennekom, C., De Bruyn, C. et al. Metabolite profiling of human muscle extracts by isotachophoresis. J Inherit Metab Dis 4, 109–110 (1981). https://doi.org/10.1007/BF02263615

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