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Identification of subnanomole amounts of PTH-amino acids by high performance thin-layer chromatography

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Summary

High performance thin-layer chromatography (HPTLC) of PTH-amino acids on 5×5 cm silica gel plates precoated with a fluorescence marker gives 10–20 fold increase in sensitivity compared to ordinary silica gel plates. Separation of PTH-Leu from PTH-Ile is easily achieved in contrast to chromatography on polyamide sheets. Only two solvent systems are required and as many as 12 samples can be chromatographed on each plate. However, if the sample is contaminated with N-phenylthiourea a third solvent system is necessary.

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  1. The Danish Institute of Protein Chemistry, 4, Venlighedsvej, DK-2970, Hørsholm, Denmark

    D. Bucher

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  1. D. Bucher
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Bucher, D. Identification of subnanomole amounts of PTH-amino acids by high performance thin-layer chromatography. Chromatographia 10, 723–725 (1977). https://doi.org/10.1007/BF02263083

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  • DOI: https://doi.org/10.1007/BF02263083

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