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Determination of thiamine and thiamine phosphates as thiochrome derivatives by reversed-phase chromatography on polystyrene packing materials

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Summary

The chromatography of thiochrome and related phosphate esters was studied on two different polystyrene packing materials.

The adsorbents were compared with respect to capacity factors, plate heights and resolution factors measured with different mixtures of phosphate buffer (pH 8.5) and methanol.

In contrast with derivatized silica, these phases were found to be stable using an alkaline mobile phase and sample. Fast separations were possible with good peak symmetry at all organic modifier concentrations.

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References

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Authors and Affiliations

  1. Laboratoire de Biochimie Générale et Comparée, Université de Liège, Place Delcour, 17, B-4020, Liege, Belgium

    J. Bontemps, L. Bettendorff, J. Lombet, G. Dandrifosse & E. Schoffeniels

  2. Laboratorium voor Organische Scheikunde, Rijksuniversiteit Gent, Krijgslaan, 281 (S. 4), B-9000, Gent, Belgium

    F. Nevejans, Y. Yang & M. Verzele

Authors
  1. J. Bontemps
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  2. L. Bettendorff
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  3. J. Lombet
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  4. G. Dandrifosse
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  5. E. Schoffeniels
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  6. F. Nevejans
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  7. Y. Yang
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  8. M. Verzele
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Bontemps, J., Bettendorff, L., Lombet, J. et al. Determination of thiamine and thiamine phosphates as thiochrome derivatives by reversed-phase chromatography on polystyrene packing materials. Chromatographia 18, 424–426 (1984). https://doi.org/10.1007/BF02262970

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  • DOI: https://doi.org/10.1007/BF02262970

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