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The influence of the amount of stationary phase and nature of solid support on retention indices

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Summary

It is shown that the retention indices of polar solutes are affected by the amount of stationary phase in the column. The retention indices increase with decreasing stationary phase loadings. The reasons for this behavior are most likely due to the existence of mixed retention mechanisms. At low amounts of stationary phases, adsorption on the support or column walls can take place. Studies were made using four supports, two of which were Chromosorb W and the other two Chromosorb P. The effects of changing the liquid phase loadings are more pronounced when using Chromosorb P.

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References

  1. E. Kováts, Helv. Chim. Acta41, 1915 (1958).

    Article  Google Scholar 

  2. L. Rohrschneider, J. Chromatogr.22, 6 (1966).

    Article  CAS  Google Scholar 

  3. W. O. McReynolds, J. Chromatogr. Sci.8, 685 (1970).

    Article  CAS  Google Scholar 

  4. J. K. Haken, Advances in Chromatography, Vol. 14,J. C. Giddings, E. Grushka, J. Cazes andP. Brown, Eds., Marcel Dekker, Inc, New York, N.Y. 1976, p. 367.

    Google Scholar 

  5. L. S. Ettre, Chromatographia6, 489 (1973);7, 39 (1974);7, 261 (1974).

    Article  CAS  Google Scholar 

  6. P. Chovin andJ. Lebbe, Séparation Immédiate et Chromatographie,J. Tranchant, Ed., G.A.M.S. Paris, France, 1962; p. 90.

    Google Scholar 

  7. G. Guiochon, Anal. Chem.36, 661 (1964).

    Article  CAS  Google Scholar 

  8. W. E. Harris andH. W. Habgood, Programmed Temperature Gas Chromatography, John Wiley & Sons, Inc., New York, N.Y., 1966; p. 153.

    Google Scholar 

  9. L. S. Ettre andK. Billeb, J. Chromatogr.30, 1 (1967).

    Article  CAS  Google Scholar 

  10. E. B. Molndr, P. Moritz andJ. Takács, J. Chromatogr.66, 205 (1972).

    Article  Google Scholar 

  11. G. D. Mitra andN. C. Saha, Chromatographia6, 93 (1973).

    Article  CAS  Google Scholar 

  12. P. H. Weiner andD. G. Howey, Anal. Chem.,46, 829 (1974).

    Article  Google Scholar 

  13. A. Hartkopf, S. Grunfeld andR. Delumyea, J. Chromatogr. Sci.12, 119 (1974).

    Article  CAS  Google Scholar 

  14. J. R. Ashes andJ. K. Haken, J. Chromatogr.118, 183 (1976).

    Article  CAS  Google Scholar 

  15. R. Kaiser, Chromatographia3, 127 (1970);3, 383 (1970).

    Article  Google Scholar 

  16. G. Schomburg, Chromatographia4, 286 (1971).

    Article  CAS  Google Scholar 

  17. J. F. K. Huber andR. G. Geruitse, J. Chromatogr.80, 25 (1973).

    Article  CAS  Google Scholar 

  18. X. Guardino, J. Albaiges, G. Feipo, R. Rodriguez-Vinals andM. Gassiot, J. Chromatogr.118, 13 (1976).

    Article  CAS  Google Scholar 

  19. J. R. Conder, D. C. Locke andH. J. Purnell, J. Phys. Chem.73, 700 (1969).

    Article  CAS  Google Scholar 

  20. L. J. Lorenz andL. B. Rogers, Anal. Chem.43, 1593 (1971).

    Article  CAS  Google Scholar 

  21. P. H. Weiner, H. L. Liao andB. L. Karger, Anal. Chem.46, 2182 (1974).

    Article  CAS  Google Scholar 

  22. T. Komaita, K. Naito andS. Takei, J. Chromatogr.114, 1 (1975).

    Article  CAS  Google Scholar 

  23. J. Klein andH. Widdecke, J. Chromatogr.129, 375 (1976).

    Article  CAS  Google Scholar 

  24. M. L. Peterson andJ. Hirsch, J. Lipid Research1, 132 (1959).

    CAS  Google Scholar 

  25. W. R. Supina, The Packed Column in Gas Chromatography, Supelco, Inc., Bellefonte, Pa. 1974.

    Google Scholar 

  26. D. M. Ottenstein, Advances in Chromatography, Vol. 3,J. C. Giddings andR. A. Keller, ed., Marcel Dekker, Inc., New York, N.Y., 1966; p. 137.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, State University of New York at Buffalo, 14214, Buffalo, N.Y., USA

    Eli Grushka & T. A. Goodwin

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  1. Eli Grushka
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  2. T. A. Goodwin
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Grushka, E., Goodwin, T.A. The influence of the amount of stationary phase and nature of solid support on retention indices. Chromatographia 10, 549–553 (1977). https://doi.org/10.1007/BF02262916

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  • DOI: https://doi.org/10.1007/BF02262916

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