Summary
The separation of some purines by thin-layer chromatography, utilizing precoated plates with Silicagel R as stationary phase and the following mobile phases: i-butanol: methylethylketone: ammonium hydroxide 25%: water (40∶25∶20∶15) and n-propanol: ammonium hydroxide 25%: water (80∶15∶5) is reported.
Visualisation of the spots has been achieved by spraying with aqueous 1% uranyl acetate and irradiating with UV both at 254 and 366 nm. The visual detection of a spot was 0.01 μg.
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Sârbu, C., Măruţoiu, C. A new detection method for purines in thin-layer chromatography. Chromatographia 20, 683–684 (1985). https://doi.org/10.1007/BF02262692
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DOI: https://doi.org/10.1007/BF02262692