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A new detection method for purines in thin-layer chromatography

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Summary

The separation of some purines by thin-layer chromatography, utilizing precoated plates with Silicagel R as stationary phase and the following mobile phases: i-butanol: methylethylketone: ammonium hydroxide 25%: water (40∶25∶20∶15) and n-propanol: ammonium hydroxide 25%: water (80∶15∶5) is reported.

Visualisation of the spots has been achieved by spraying with aqueous 1% uranyl acetate and irradiating with UV both at 254 and 366 nm. The visual detection of a spot was 0.01 μg.

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Authors and Affiliations

  1. Faculty of Mechanical Engineering, Polytechnic Institute, 3400, Cluj-Napoca, România

    C. Sârbu

  2. Chemical Institute, 3400, Cluj-Napoca, România

    C. Măruţoiu

Authors
  1. C. Sârbu
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  2. C. Măruţoiu
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Sârbu, C., Măruţoiu, C. A new detection method for purines in thin-layer chromatography. Chromatographia 20, 683–684 (1985). https://doi.org/10.1007/BF02262692

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  • DOI: https://doi.org/10.1007/BF02262692

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