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Esterification of amino acids to n-butyl and isobutyl esters in aqueous solutions for their gas chromatographic analysis

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Summary

Amino acids have been esterified with n-butanol and isobutanol in the presence of perchloric acid. It has been established that in the concentration range of [H2O]/[n-BuOH]=0.10–0.40 esterification can be utilized for the determination of alanine, glycine, valine, leucine, proline, methionine, aspartic acid, phenylalanine, tyrosine, glutamic acid, lysine, arginine, histidine and cystine. In the case of [H2O]/[i-BuOH] =0.05–0.20 the esterification yield decreases from 100% to 70%, in proportion to the water content.

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Authors and Affiliations

  1. Institute of Inorganic and Analytical Chemistry, L. Eötvös University, Muzeum krt. 4/B, 1088, Budapest, Hungary

    I. Molnár-Perl, V. Fábián-Vonsik & M. Szakács-Pintér

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  1. I. Molnár-Perl
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  2. V. Fábián-Vonsik
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  3. M. Szakács-Pintér
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Molnár-Perl, I., Fábián-Vonsik, V. & Szakács-Pintér, M. Esterification of amino acids to n-butyl and isobutyl esters in aqueous solutions for their gas chromatographic analysis. Chromatographia 20, 652–656 (1985). https://doi.org/10.1007/BF02262686

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  • DOI: https://doi.org/10.1007/BF02262686

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