Summary
Under our climatical conditionsMicrodochium nivale is the main pathogen which causes snow mould in grasses. Like manyFusarium species,M. nivale is able to produce toxins like Deoxynivalenol (DON). In vitro selection with callus cultures from two populations of perennial ryegrass and DON as selective agent was carried out in a one step (subculture) or two step procedure (induction plus subculture). The regenerants from in vitro selection and a seed derived control from the two populations were artificially inoculated withM. nivale. Significant improvement of snow mould tolerance in the high DON variant could be found. Additionally, a toxin test was established which allows to screen large numbers of mature embryos without laborious callus culture. The sexual progenies (R1) of the regenerants were subjected to both the snow mould and the toxin test. Toxin and pathogen resistance were not always in good accordance. The application of both tests in practical plant breeding is discussed.
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Posselt, U.K., Altpeter, F. Improvement of snow mould resistance by conventional and in vitro techniques. Euphytica 77, 251–255 (1994). https://doi.org/10.1007/BF02262638
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DOI: https://doi.org/10.1007/BF02262638