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Influence of the thermal modification of controlled porosity glass on the affinity chromatography of fungal proteins

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Summary

The properties and the coverage density of chemically bonded phases depend among others on the properties of their supports. Controlled-porosity glasses (CPG) are materials used as a support of bio-active ligands. Their network mainly consist of SiO2 as well as of a small amount of B2O3 and Na2O. The characteristic feature of porous glasses is the possibility of a change in the surface boron concentration leading to the change of the properties of their surface.

This paper deals with the results of the separation of fungal proteins on packings consisting of vanillin bonded to CPG with different boron concentration on its surface. It appears from the data obtained that the changes in the affinity of the fungal proteins to the packing are related to the extend of the thermal treatment of the CPG. The protein fractions separated by chromatography were confirmed to be homogeneous by poly-acryloamide gel disc electrophoresis.

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Authors and Affiliations

  1. Department of Chemical Physics, Maria Curie-Skłodowska University, 20-031, Lublin, Poland

    A. L. Dawidowicz

  2. Department of Biochemistry, Maria Curie-Skłodowska University, 20-031, Lublin, Poland

    J. Łobarzewski

Authors
  1. A. L. Dawidowicz
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  2. J. Łobarzewski
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Dawidowicz, A.L., Łobarzewski, J. Influence of the thermal modification of controlled porosity glass on the affinity chromatography of fungal proteins. Chromatographia 18, 389–392 (1984). https://doi.org/10.1007/BF02262487

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  • DOI: https://doi.org/10.1007/BF02262487

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