Summary
The technique of an internal pH gradient induced by the sample is applied to the separation of proteins by liquid chromatography. Compatibility of the method with microcolumns is demonstrated and examples of separations on different types of sorbents are given.
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Janeček, M., Šalamoun, J. & Šlais, K. Sample-induced internal pH gradients in microcolumn liquid chromatography of proteins. Chromatographia 32, 61–64 (1991). https://doi.org/10.1007/BF02262468
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DOI: https://doi.org/10.1007/BF02262468