Skip to main content
Log in

A comparative study of injection techniques in high-performance liquid chromatography

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

The performance of injection valves with both curtain- and single-flow column couplings has been evaluated using reverse-phase octadecylsilane columns and a standard test mixture. The results are compared with those obtained from septum and stopped-flow syringe injections.

Stopped-flow valve injection has been examined similarly. Configurations and couplings which give optimum efficiency and reproducibility are recommended.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. J. H. Knox (ed.), High-Performance Liquid Chromatography, Edinburgh University Press, 1978, Ch. 8.

  2. J. J. N. Webber andE. H. McKerrell, J. Chromatogr.122, 259 (1976).

    Google Scholar 

  3. G. B. Cox, C. R. Loscombe, M. J. Slucutt, K. Sugden andJ. A. Upfield, J. Chromatogr.117, 259 (1976).

    Google Scholar 

  4. R. M. Cassidy andR. W. Frei, Anal. Chem.44, 2250 (1972).

    Google Scholar 

  5. P. A. Bristow, Liquid Chromatography in Practice, HETP (1976).

  6. J. J. Kirkland, W. W. Yau, K. J. Stoklosa andC. H. Dilks, J. Chromatogr. Sci.15, 303 (1977).

    Google Scholar 

  7. Ref. [1],J. H. Knox (ed.), High-Performance Liquid Chromatography, Edinburgh University Press, 1978, Ch 13, p. 174.

  8. Ref. [1],J. H. Knox (ed.), High-Performance Liquid Chromatography, Edinburgh University Press, 1978, CH 2, p. 10.

  9. J. H. Done, J. Chromatogr.125, 43 (1976).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kelsey, R.J., Loscombe, C.R. A comparative study of injection techniques in high-performance liquid chromatography. Chromatographia 12, 713–715 (1979). https://doi.org/10.1007/BF02262448

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02262448

Key Words

Navigation