, Volume 31, Issue 9–10, pp 507–511 | Cite as

Packing meterials with surface barriers, controlled distribution and topography of ligands for sample clean-up and analysis of biologically active solutes in HPLC

  • K. K. Unger
Review Paper


Various types of, so-called, restricted access packings have been developed for sample clean-up and analysis of biologically active solutes by means of HPLC. The unique feature of these packings is that they prevent the access of matrix components such as protein whilst selectively retaining the drug components and their metabolites. This results from the fact that the particles of the packings possess a surface barrier for large solutes at the external or internal surface area and exhibit a controlled gradient in the chemical surface composition. They are grouped, according to their particle structures, into internal-surface reversed phases (ISRP), shielded hydrophobic phases (SHP), semi-permeable surfaces (SPS), dual zone phases (DZP) and mixed functional phases (MFP). The structural characteristics and the chromatographic properties of these packings are reviewwed, especially with regard to their use in the assaying of drugs.

Key Words

Column liquid chromatography Restricted access packings Packings with surface barriers Sample clean-up of biologically active analytes 


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  1. [11.
    R. W. Frei, K. Zech, Selective Sample Handling and Detection in High-Performance Liquid Chromatography, J. Chromatogr. Library, Vol. 39 A and B, Elsevier, Amsterdam, 1988 and 1989.Google Scholar
  2. [2]
    D. Westerlund, Chromatographia,24, 155 (1987).Google Scholar
  3. [3]
    J. A. Perry, L. J. Glunz, T. J. Szcerba, J. D. Rateike, LC GC Internat.,3, No. 12, 10 (1990).Google Scholar
  4. [4]
    J. Haginaka, Trends in Analytical Chemistry,10, 17 (1991).Google Scholar
  5. [5]
    H. Yoshida, I. Morita, T. Masujima, H. Imai, Chem. Pharm. Bull.,30, 2287 (1982).Google Scholar
  6. [6]
    H. Yoshida, I. Morita, G. Tamai, T. Matsujima, T. Tsuru, N. Takai, H. Imai, Chromatographia,19, 466 (1985).Google Scholar
  7. [7]
    H. Yoshida, K. Takano, I. Morita, T. Matsujima, H. Imai, Jap. J. Clin. Chem.,12, 312 (1983).Google Scholar
  8. [8]
    I. H. Hagestam, T. C. Pinkerton, Anal. Chem.,57, 1757 (1985).Google Scholar
  9. [9]
    S. E. Cook, T. C. Pinkteton, J. Chroamtogr.,368, 233 (1986).Google Scholar
  10. [10]
    I. H. Hagestam, T. C. Pinkerton, J. Chromatogr.,351, 239 (1986).Google Scholar
  11. [11]
    S. E. Cook, Ph.D. Thesis, Department of Biochemistry, Purdue University, West Lafayette, IN, USA, 1988.Google Scholar
  12. [12]
    I. H. Hagestam, T. C. Pinkerton, J. Chromatogr.,368, 77 (1986).Google Scholar
  13. [13]
    J. A. Perry, J. Liq. Chromatogr.,13, 1047 (1990).Google Scholar
  14. [14]
    J. Haginaka, N. Yasuda, J. Wakai, H. Matsunaga, H. Yasuda, Y. Kimara, Anal. Chem.,61, 2445 (1989).Google Scholar
  15. [15]
    D. E. Williams, P. M. Kabra, Anal. Chem.,62, 807 (1990).Google Scholar
  16. [16]
    K. Kimata, R. Tsuboi, K. Hosoya, N. Tanaka, T. Araki, J. Chromatogr.,515, 73 (1990).Google Scholar
  17. [17]
    K. S. Boos, B. Wilmers, J. Lintelmann, P. Marth, A. Walfort, Keistdagar i Analytisk Kemi June 1990, Lund, Sweden.Google Scholar
  18. [18]
    V.A. Davankov, M. P. Tsyurupa, Reactive Polymers,13, 27 (1990).Google Scholar
  19. [19]
    V. A. Davankov, M. P. Tsyurupa, Pure Appl. Chem.,61, 1881 (1989).Google Scholar
  20. [20]
    T. J. Ward, D. W. Armstrong, J. Liq. Chromatogr.,9, 407 (1986).Google Scholar
  21. [21]
    D. W. Armstrong, W. Li, Chromatographia,21, 43 (1987).Google Scholar
  22. [22]
    C. P. Desilets, F. E. Regnier, 12th International Symposium on Column Liquid Chromatography, Washington, D.C., June 1988, poster presentation No. W-P-326.Google Scholar
  23. [23]
    G. J. Glunz, J. A. Perry, B. K. Invergo, H. Wagner, T. J.Szczerba, J. D. Rateike, Pittsburgh Conference on Analytical Chemistry, New York, NY, March 1990, paper No. 490.Google Scholar
  24. [24]
    D. J. Gisch, B. T. Hunter, B. Feibush, J. Chromatogr.,433, 264 (1988).Google Scholar
  25. [25]
    D. J. Gisch, B. Feibush, B. T. Hunter, T. L. Ascah, Biochromatogr.,4, 206 (1989).Google Scholar
  26. [26]
    B. Feibush, C. T. Santasania, J. Chromatogr., in print.Google Scholar
  27. [27]
    R. A. Majors, LC GC Internat.,3, No. 4, 12 (1990).Google Scholar
  28. [28]
    Th. R. Floyd, R. A. Hartwick, Mixed Interaction Stationary Phases in HPLC, inCs. Horváth (ed.), HPLC: Advances and Perspectives, Academic Press, London 1986, Vol. 4, pp. 45–87.Google Scholar
  29. [29]
    J. Haginaka, J. Wakai, Anal. Chem.,62, 997 (1990).Google Scholar
  30. [30]
    J. Haginaka, J. Wakai, Chromatographia,29, 223 (1990).Google Scholar
  31. [31]
    J. Haginaka, J. Wakai, H. Yasuda, J. Chromatogr.,535, 163 (1990).Google Scholar
  32. [32]
    A. Shibukawa, T. Nakagawa, N. Nishimura, M. Miyake, H. Tanaka, Chem. Pharm. Bull.,37, 702 (1989).Google Scholar
  33. [33]
    A. Shibukawa, N. Nishimura, K. Nomura, Y. Kuroda, T. Nakagawa, Chem. Pharm. Bull.,38, 443 (1990).Google Scholar
  34. [34]
    A. Shibukawa, M. Nago, Y. Kuroda, T. Nakagawa, Anal. Chem.,62, 62 (1990).Google Scholar
  35. [35]
    S. J. Rainbow, C. D. Dawson, T. R. Tickner, Ann. Clin. Biochem.,26, 527 (1989).Google Scholar
  36. [36]
    C. M. Dawson, T. W. M. Wang, S. J. Rainbow, T. R. Tickner, Ann. Clin. Biochem.,25, 661 (1988).Google Scholar
  37. [37]
    T. Niwa, N. Takeda, A. Tatmatsu, K. Maeda, Clin. Chem.,34, 2264 (1988).Google Scholar
  38. [38]
    F. Tagliaro, R. Dorizzi, A. Frigerio, M. Marigo, Clin. Chem.,36, 113 (1990).Google Scholar
  39. [39]
    N. D. Atherton, Clin. Chem.,35, 975 (1989).Google Scholar
  40. [40]
    L. T. Karolak, R. Farinotti, M. J. Bossant, A. Dauphin, J. Pharm. Clin.,7, 543 (1988).Google Scholar
  41. [41]
    T. C. Pinkerton, J. A. Perry, J. D. Rateike, J. Chromatogr.,367, 412 (1986).Google Scholar
  42. [42]
    T. Ohsnima, I. Johno, T. Hasegawa, S. Kitazawa, J. Liq. Chromatogr.,11, 3457 (1988).Google Scholar
  43. [43]
    R. H. Pullen, C. M. Kennedy, M. A. Curtis, J. Chromatogr.,434, 271 (1988).Google Scholar
  44. [44]
    T. Nakagawa, A. Shibukawa, N. Shimono, T. Kawashima, H. Tanaka, J. Chromatogr.,420, 297 (1987).Google Scholar
  45. [45]
    L. E. Mathes, G. Muschik, L. Demby, P. Polas, D. W. Mellini, H. J. Issaq, R. Sams, J. Chromatogr.,432, 346 (1987).Google Scholar
  46. [46]
    J. Haginaka, N. Yasuda, J. Wakai, H. Matsunaga, H. Yasuda, Y. Kimura, Anal. Chem.,61, 2445 (1989).Google Scholar
  47. [47]
    K. S. Boos, B. Wilmers, R. Sauerbrey, E. Schlimme, Chromatographia,24, 363 (1987).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1991

Authors and Affiliations

  • K. K. Unger
    • 1
  1. 1.Institut für Anorganische Chemie und analytische ChemieJohannes-Gutenberg-UniversitätMainzGermany

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