, Volume 33, Issue 11–12, pp 525–532 | Cite as

On-line monitoring of fermentation processes by ultrafiltration and column liquid chromatography

  • N. C. van de Merbel
  • I. M. Kool
  • H. Lingeman
  • U. A. Th. Brinkman
  • A. Kolhorn
  • L. C. de Rijke


An automated on-line monitoring system for low-molecular-weight compounds (e.g. sugars) during fermentation processes is described. The applicability of cross-flow ultrafiltration, coupled on-line with high-performance liquid chromatography, is evaluated. Various detection modes for the determination of the test compounds lactose, glucose and fructose) in a complex fermentation broth are compared. In addition, the influence of some system parameters on the performance of several ultrafiltration modules for the removal of cellular and macromolecular broth constituents is investigated. A method for the fully automated determination of sugars during anE. coli batch culture in a brain-heart infusion medium is presented. With the present system five analyses can be performed every hour, using only 35μl of sample, without significant deterioration of the ultrafiltration process. Contamination by external micro-organisms was not observed and good correlation with an off-line method was found.

Key Words

Column liquid chromatography Ultrafiltration Fermentation monitoring Sugars 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1992

Authors and Affiliations

  • N. C. van de Merbel
    • 1
  • I. M. Kool
    • 1
  • H. Lingeman
    • 1
  • U. A. Th. Brinkman
    • 1
  • A. Kolhorn
    • 2
  • L. C. de Rijke
    • 3
  1. 1.Department of Analytical ChemistryFree UniversityAmsterdamThe Netherlands
  2. 2.Bio-FloGlasgowU.K.
  3. 3.Pharmacia LKB Biotechnologie NederlandWoerdenThe Netherlands

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