Summary
A simple rapid procedure is described for estimating optimum compositions of ternary mobile phase mixtures for the separation of samples by reversed-phase liquid chromatography (RPLC). Retention data in two iso-eluotropic binary mobile phase mixtures (mixtures with equal retention times) are required to initiate the procedure. The logarithm of the capacity factor is assumed to vary linearly with the composition of isoeluotropic ternary mixtures formed by mixing the two limiting binaries. Using the product of resolution factors of adjacent peaks as the criterion, an optimum ternary composition is then calculated. After a chromatogram has been obtained with the predicted optimum ternary mobile phase, the procedure is repeated until no further improvement can be achieved. Examples of the application of the present procedure are described to illustrate its effectiveness.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
P. J. Schoenmakers, H. A. H. Billiet, L. de Galan, J. Chromatogr.205, 13 (1981).
N. A. Parris, J. Chromatogr.149, 615 (1978).
N. A. Parris, J. Chromatogr.157, 161 (1978).
P. J. Schoenmakers, H. A. H. Billiet, L. de Galan, Chromatographia15, 205 (1982).
L. R. Snyder, J. Chrom. Sci.16, 223 (1978).
R. Tijssen, H. A. H. Billiet, P. J. Schoenmakers, J. Chromatogr.122, 185 (1976).
S. R. Bakalyar, R. McIlwrick, E. Roggendorf, J. Chromatogr.142, 353 (1977).
P. J. Schoenmakers, H. A. H. Billiet, L. de Galan, J. Chromatogr.185, 179 (1979).
H. A. H. Billiet, P. J. Schoenmakers, L. de Galan, J. Chromatogr.218, 443 (1981).
P. J. Schoenmakers, H. A. H. Billiet, L. de Galan, J. Chromatogr.218, 261 (1981).
J. L. Glajch, J. H. Kirkland, K. M. Squire, J. M. Minor, J. Chromatogr.199, 57 (1980).
A. C. J. H. Drouen, H. A. H. Billiet, P.J. Schoenmakers, L. de Galan, Chromatographia, in press.
R. J. Laub, J. H. Purnell, J. Chromatogr.112, 71 (1975).
R. J. Laub, J. H. Purnell, Anal. Chem.48 (6), 799 (1976).
R. J. Laub, J. h. Purnell, P. S. Williams, J. Chromatogr.134, 249 (1977).
P. Jones, C. A. Wellington, J. Chromatogr.213, 357 (1982).
F. Lottspeich, Proceedings Königsteiner Chromatographic Tage, Salzburg 1980, pp. 274–280.
P. Jandera, J. Churácek, H. Colin, J. Chromatogr.214, 35 (1981).
L. R. Snyder, J. J. Kirkland, Introduction to modern liquid chromatography, 2nd edition, Wiley New York, 1979, Chapter 2.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schoenmakers, P.J., Drouen, A.C.J.H., Billiet, H.A.H. et al. A simple procedure for the rapid optimization of reversed-phase separations with ternary mobile phase mixtures. Chromatographia 15, 688–696 (1982). https://doi.org/10.1007/BF02261886
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02261886