Summary
A high-pressure liquid chromatographic assay capable of quantitating subnanogram per milliliter concentrations of propranolol in microliter volume of serum was developed. Propranolol was extracted from serum with hexane at pH 9.7. Further sample clean up was achieved by back extraction. Separation of propranolol from its metabolites and normal serum constituents was performed on 10 μm C18 reversed-phase column packing. High sensitivity was attained with fluorescence detection using a low UV excitation wavelength. This assay is suitable for use in pharmacokinetic studies involving small laboratory animals.
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Terao, N., Shen, D.D. A sensitive high-pressure liquid chromatographic method for the determination of propranolol in microliter serum samples. Chromatographia 15, 685–687 (1982). https://doi.org/10.1007/BF02261885
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DOI: https://doi.org/10.1007/BF02261885