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Chromatographic investigations into the interaction between proteins and polymer matrices

II. Chromatography of serum proteins on substituted agarose gels

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Summary

To study the adsorption behaviour of serum proteins on different chemical moieties as present on the surface of polymers, a chromatographic method was developed: the relative adsorptivity of the proteins on substituted Sepharose 4B gels [1] was determined by measurng their elution volumes under standnardised conditions. The reseults show that the interaction between the proteins and gels is dependent on both and difers markedly in strength.

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Abbreviations

vi :

internal volume of gels

vo :

void volume

vt :

total volume of packed column

ve :

elution volume for the particular protein

Ki :

distribution coefficient for protein between gel (i) and mobile phase (buffer)

$$K_i = \frac{{V_e - V_0 }}{{V_i }}$$

References

  1. Chromatographic Investigations into the Interaction between Proteins and Pollymer Mtricese. I. Agarose Gels with Substituents of Different Length and Hydrophobicity, Chromatographia10, 129 (1977).

    Google Scholar 

  2. R. M. Wheaton, W. L. Baumann, Ann. N.Y. Acad. Sci.57, 159 (1953).

    Google Scholar 

  3. In:I. Witit, Biochemie der Blutgerinnung und Fibrinolyse, Verlag Chemie, D-6940 Weinheim, 1957.

    Google Scholar 

  4. C. L. Lowe, P. D. G. Dean, Affinity Chromatography, J. Wiley & Sons, London, 1974, p. 18.

    Google Scholar 

  5. ibid., p. 27–27.

    Google Scholar 

  6. B. M. J. Hofstee, Biochem., Biophys. Res. Commun.50, 751 (1973).

    Google Scholar 

  7. M. W. Davey, E. Sulkowski, W. A. Carter, J. Biol. Chem.251, 7620 (1976).

    Google Scholar 

  8. B. M. J. Hofstee, Prep. Biochem.5, 7 (1975).

    Google Scholar 

  9. C. Tanford, The Hydrophobic Effects, Wiley-Interscience Public., 1973.

  10. B. M. J. Hofstee, Polymer Preprints15, 311 (1975).

    Google Scholar 

  11. C. C. Rigelow, J. Theor. Biol.16, 187 (1967).

    Google Scholar 

  12. J. Rosengren, S., Påhlmann, M. Glad, S. Hjértén, Biophys., Biochim. Acta412, 51 (1975).

    Google Scholar 

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Authors and Affiliations

  1. Abteilung Angewandte Chemie, Battelle-Institute e. V., Am Römerhof 36, D-6000, Frankfurt a. M., Germany Federal Republic

    W. Kißing & R. H. Reiner

Authors
  1. W. Kißing
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  2. R. H. Reiner
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Kißing, W., Reiner, R.H. Chromatographic investigations into the interaction between proteins and polymer matrices. Chromatographia 11, 83–88 (1978). https://doi.org/10.1007/BF02261768

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  • DOI: https://doi.org/10.1007/BF02261768

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