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On-line fluorescence detection in HPLC: A high sensitive technique for the measurement of low enzyme activities: The case of tryptophan-hydroxylase

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Summary

The use of on-line fluorescence detection after high-performance liquid chromatographic separation permits the direct quantitative analysis of the reaction mixture of enzymes with low activity. The application to the determination of tryptophan-hydroxylase in vitro as well as in vivo is described. The whole system has a detection limit of 0.1 μg 5-hydroxytryptophan formed/hour/gram of brains.

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Authors and Affiliations

  1. Department of Biochemistry, Free University Brussels, Belgium

    F. Geeraerts, L. Schimpfessel & R. Crokaert

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  1. F. Geeraerts
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  2. L. Schimpfessel
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  3. R. Crokaert
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Geeraerts, F., Schimpfessel, L. & Crokaert, R. On-line fluorescence detection in HPLC: A high sensitive technique for the measurement of low enzyme activities: The case of tryptophan-hydroxylase. Chromatographia 15, 449–450 (1982). https://doi.org/10.1007/BF02261606

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  • DOI: https://doi.org/10.1007/BF02261606

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