Summary
The responding signals of eluted components can be enhanced by using high performance small volume columns with long lengths generating more plates than required for a separation with a preset resolution in combination with instruments which show very small external bandbroadening. The excess number of plates is then consequently erased by maximizing the injection volume of the sample until the preset resolution is reached. Equations, describing the dilution, relative signal enhancement and maximum injection volume as a function of the process parameters are derived and experimentally verified. Theory and experiment are in agreement only if variances of response functions are calculated as their second normalized central moments.
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Lauer, H.H., Rozing, G.P. The selection of optimal conditions in HPLC II. The influence of column dimensions and sample size on solute detection. Chromatographia 15, 409–413 (1982). https://doi.org/10.1007/BF02261599
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DOI: https://doi.org/10.1007/BF02261599