Chromatographia

, Volume 13, Issue 7, pp 447–450 | Cite as

Analysis of the fusariotoxins zearalenone and vomitoxin (deoxynivalenol) in human foods and animal feeds by high-performance liquid chromatography (HPLC)

  • H. Schweighardt
  • J. Böhm
  • A. M. Abdelhamid
  • J. Leibetseder
  • M. Schuh
  • E. Glawischnig
Originals

Summary

HPLC procedures for analyses of the fusariotoxins zearalenone and vomitoxin in individual food- and feedstuffs as well as in mixed feed are described. Zearalenone is separated on a column with polar stationary phase (25 cm × 4.6 mm i.d., particle size 7 μm), eluted with a chloroform-isooctane (75/25, v/v)+1.5% methanol mixture and detected fluorometrically. The quantitative determination was possible in all analyzed samples with a detection limit of 2μg/kg with 70–80% recovery. Vomitoxin is fractionated by HPLC (C 18 1 column, 25 cm×4 mm i.d., 5 μm particle size) with water-methanol (60/40, v/v) mobile phase and determined by combining GLC or TLC with UV detection. The detection limit in mixed feed with interfering substances was 25 μg/kg (recovery 25–35%). The separation by HPLC makes preparation of pure vomitoxin possible. The described methods are fast, simple and low cost and are suitable for routine analyses.

Key Words

HPLC methods Mycotoxins Zearlenone Vomitoxin (Deoxynivalenol) 

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References

  1. [1]
    H. M. Müller, Daten und Dokumente zum Umweltschutz, Sonderreihe Umwelttagung23, 153 (1979).Google Scholar
  2. [2]
    Y. Ueno in “Mycotoxins in Human and Animal Health”,J. V. Rodricks, C. W. Hesseltine andM. A. Mehlman, ed., Pathotox Publisher, Inc., Gustav Fischer Verlag, Stuttgart-New York, 1977; p. 189.Google Scholar
  3. [3]
    H. M. Müller, Übers. Tierernähr.6, 265 (1978).Google Scholar
  4. [4]
    H. Lew, E. Müllner R. Hager andM. Gregor, Journal für landw. Forschung30, 309 (1979).Google Scholar
  5. [5]
    C. L. Holder, C. R. Nony andM. C. Bowman, Journal of the AOAC60, 272 (1977).Google Scholar
  6. [6]
    P. M. Scott, T. Panalaks, S. Kanhere andW. F. Miles, Journal of the AOAC61, 593 (1978).Google Scholar
  7. [7]
    T. E. Möller andE. Josefsson, Journal of the AOAC61, 789 (1978).Google Scholar
  8. [8]
    H. Schweighardt, J. Böhm andJ. Leibetseder, Zeitschrift für Tierphysiologie, Tierernährung und Futtermittelkunde41, 39 (1978).Google Scholar
  9. [9]
    H. J. Mirocha, B. Schauerhamer andS. V. Pathre, Journal of the AOAC57, 1104 (1974).Google Scholar
  10. [10]
    H. Engelhardt “Hochdruck-Flüssigkeits-Chromatographie”, Zweite Auflage, Springer Verlag, Berlin-Heidelberg-New York, 1977; p. 48.Google Scholar
  11. [11]
    L. Stoloff, Journal of the AOAC62, 356 (1979).Google Scholar
  12. [12]
    W. F. O. Marasas, N. P. J. Kriek, S. J. van Rensburg, M. Steyn andG. C. van Schalkwyk, South African Journal of Science73, 346 (1977).Google Scholar
  13. [13]
    R. F. Vesonder, A. Ciegler, R. F. Rogers, K. A. Burbridge, R. J. Bothast andA. H. Jensen, Appl. Environ. Microbiol36, 885 (1978).PubMedGoogle Scholar
  14. [14]
    R. F. Vesonder, A. Ciegler, A. H. Jensen, W. K. Rohwedder andD. Weisleder, Appl. Environ. Microbiol.31, 280 (1976).PubMedGoogle Scholar
  15. [15]
    R. F. Vesonder, A. Ciegler andA. H. Jensen, Appl. Environ. Microbiol.34, 105 (1977).PubMedGoogle Scholar
  16. [16]
    D. M. Forsyth, T. Yoshizawa, N. Morooka andJ. Tuite, Appl. Environ. Microbiol.34, 547 (1977).PubMedGoogle Scholar
  17. [17]
    S. V. Pathre andC. J. Mirocha, Appl. Environ. Microbiol.35, 992 (1978).PubMedGoogle Scholar
  18. [18]
    H. M. Stahr, A. A. Kraft andM. Schuh, Appl. Spectroscopy33, 294 (1979).CrossRefGoogle Scholar
  19. [19]
    L. Stoloff, Journal of the AOAC60 (1977).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1980

Authors and Affiliations

  • H. Schweighardt
    • 1
  • J. Böhm
    • 1
  • A. M. Abdelhamid
    • 1
  • J. Leibetseder
    • 1
  • M. Schuh
    • 2
  • E. Glawischnig
    • 2
  1. 1.Institut für ErnährungVeterinärmedizinische Universität WienWienAustria
  2. 2.II. Medizinische Universitätsklinik für KlauentiereVeterinärmedizinische Universität WienWienAustria

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